Benefiting from deep learning, defocus blur detection (DBD) has made prominent progress. Existing DBD methods generally study multiscale and multilevel features to improve performance. In this article, from a different perspective, we explore to generate confrontational images to attack DBD network. Based on the observation that defocus area and focus region in an image can provide mutual feature reference to help improve the quality of the confrontational image, we propose a novel mutual-referenced attack framework. Firstly, we design a divide-and-conquer perturbation image generation model, where the focus region attack image and defocus area attack image are generated respectively. Then, we integrate mutual-referenced feature transfer (MRFT) models to improve attack performance. Comprehensive experiments are provided to verify the effectiveness of our method. Moreover, related applications of our study are presented, e.g., sample augmentation to improve DBD and paired sample generation to boost defocus deblurring.