Not only is human T cell development characterized by unique changes in surface marker expression, but it also requires specific growth factors and conditions to mimic and study T cell development in vitro. In this chapter, we provide an overview of the specific aspects that need attention when performing T cell differentiation cultures with human hematopoietic and T cell progenitors.
Keywords: Artificial thymic organoid; Gene perturbation; Hematopoietic stem cell; Human; In vitro; Lineage differentiation; OP9 co-culture; T cell development; Viral transduction.
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