Isolation and Compositional Analysis of Glycosaminoglycans

Stephanie Archer-Hartmann; Lauren E Pepi; Christian Heiss; Parastoo Azadi

doi:10.1007/978-1-0716-2835-5_14

Isolation and Compositional Analysis of Glycosaminoglycans

Methods Mol Biol. 2023:2597:177-186. doi: 10.1007/978-1-0716-2835-5_14.

Authors

Stephanie Archer-Hartmann¹, Lauren E Pepi¹, Christian Heiss¹, Parastoo Azadi²

Affiliations

¹ University of Georgia, Complex Carbohydrate Research Center, Athens, GA, USA.
² University of Georgia, Complex Carbohydrate Research Center, Athens, GA, USA. azadi@ccrc.uga.edu.

PMID: 36374422
DOI: 10.1007/978-1-0716-2835-5_14

Abstract

The compositional and structural analysis of GAGs is challenging due to their heterogenous structures. Strong anion exchange (SAX) HPLC can aid in the compositional analysis of GAGs and can separate complex mixtures based on charge and degree of sulfation. Herein we describe the digestion and release of GAGs from tissue, and the compositional analysis using SAX-HPLC.

Keywords: GlycosaminoglycanHeparan sulfate; Heparin; Liquid chromatography; Strong anion exchange; Sulfation; Ultraviolet detection.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Anions
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Glycosaminoglycans* / chemistry

Substances

Glycosaminoglycans
Anions

Grants and funding

R24 GM137782/GM/NIGMS NIH HHS/United States