Comparison of the inactivation capacity of various UV wavelengths on SARS-CoV-2

Ryosuke Matsuura; Chieh-Wen Lo; Takayo Ogawa; Masaru Nakagawa; Masami Takei; Yasunobu Matsumoto; Satoshi Wada; Yoko Aida

doi:10.1016/j.bbrep.2022.101379

Comparison of the inactivation capacity of various UV wavelengths on SARS-CoV-2

Biochem Biophys Rep. 2022 Dec:32:101379. doi: 10.1016/j.bbrep.2022.101379. Epub 2022 Nov 7.

Authors

Ryosuke Matsuura^{1

2}, Chieh-Wen Lo^{1

2

3}, Takayo Ogawa⁴, Masaru Nakagawa², Masami Takei², Yasunobu Matsumoto^{1

3}, Satoshi Wada^{2

4}, Yoko Aida^{1

2

3}

Affiliations

¹ Laboratory of Global Infectious Diseases Control Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
² Division of Hematology and Rheumatology, Department of Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-kamichou, Itabashi-ku, Tokyo, 173-8610, Japan.
³ Laboratory of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
⁴ Photonics Control Technology Team, RIKEN Center for Advanced Photonics, Hirosawa 2-1, Wako, 351-0198, Japan.

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused a worldwide pandemic. Ultraviolet (UV) is regarded as a very powerful tool against SARS-CoV-2. However, the inactivating effects of different UV wavelengths on SARS-CoV-2 under the same conditions have hardly been compared. Here, we showed that SARS-CoV-2 cultured in Dulbecco's modified Eagle's medium and 2% fetal bovine serum was efficiently inactivated by irradiation with 222, 254, and 265 wavelengths UV, but not at 308 nm. In addition, it was revealed that UV absorption by DMEM-2% FBS is very efficient at 222 nm. Our results present potentially important information for selecting the optimum UV wavelength according to the application.

Keywords: COVID-19; Inactivation; SARS-CoV-2; UV.