Two P450 genes, CYP6SN3 and CYP306A1, involved in the growth and development of Chilo suppressalis and the lethal effect caused by vetiver grass

Yanhui Lu; Qi Bai; Qiang Li; Xusong Zheng; Junce Tian; Jiawen Guo; Hongxing Xu; Zhongxian Lu

doi:10.1016/j.ijbiomac.2022.11.087

Two P450 genes, CYP6SN3 and CYP306A1, involved in the growth and development of Chilo suppressalis and the lethal effect caused by vetiver grass

Int J Biol Macromol. 2022 Dec 31;223(Pt A):860-869. doi: 10.1016/j.ijbiomac.2022.11.087. Epub 2022 Nov 11.

Authors

Yanhui Lu¹, Qi Bai¹, Qiang Li¹, Xusong Zheng¹, Junce Tian¹, Jiawen Guo¹, Hongxing Xu², Zhongxian Lu³

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
² State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. Electronic address: xuhongxing@mail.zaas.ac.cn.
³ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. Electronic address: lvzx@mail.zaas.ac.cn.

PMID: 36372110
DOI: 10.1016/j.ijbiomac.2022.11.087

Abstract

Chilo suppressalis is a widely distributed pest occurring in nearly all paddy fields, which has developed high level resistance to different classes of insecticides. Vetiver grass has been identified as a dead-end trap plant for the alternative control of C. suppressalis. In this study, two cytochrome P450 monooxygenase (P450) genes, CsCYP6SN3 and CsCYP306A1, were identified and characterized, which are expressed at all developmental stages, with the highest expression in the midguts and fat bodies of 3rd instar larvae. Vetiver significantly inhibited the expression levels of CsCYP6SN3 and CsCYP306A1 in 3rd larvae after feeding. RNA interference showed that silencing CsCYP6SN3 and CsCYP306A1 genes dramatically reduced the pupation rate and pupa weight. Feeding on vetiver after silencing CsCYP6SN3 and CsCYP306A1 led to higher mortality compared with feeding on rice. In conclusion, these findings indicated that the expression levels of CsCYP6SN3 and CsCYP306A1 were associated with the lethal effect of vetiver against C. suppressalis larvae and functional knowledge about these two detoxification genes could provide new targets for agricultural pest control.

Keywords: Cytochrome P450 monooxygenase; Lethality; Vetiver grass.

MeSH terms

Animals
Chrysopogon*
Insecticides* / pharmacology
Larva
Moths*
Oryza* / genetics
Pupa / genetics

Substances

Insecticides