The association of modified QuEChERS and DLLME to offer high analytical detectability to assess residual depletion profile of erythromycin in fish

Sarah Chagas Campanharo; Agnaldo Fernando Baldo da Silva; Anaïs Bleuzen; Jonas Joaquim Mangabeira da Silva; Lucas Victor Pereira de Freitas; Inácio Mateus Assane; Fabiana Pilarski; Jonas Augusto Rizzato Paschoal

doi:10.1016/j.foodchem.2022.134852

The association of modified QuEChERS and DLLME to offer high analytical detectability to assess residual depletion profile of erythromycin in fish

Food Chem. 2023 Mar 30;405(Pt A):134852. doi: 10.1016/j.foodchem.2022.134852. Epub 2022 Nov 10.

Authors

Sarah Chagas Campanharo¹, Agnaldo Fernando Baldo da Silva¹, Anaïs Bleuzen², Jonas Joaquim Mangabeira da Silva¹, Lucas Victor Pereira de Freitas¹, Inácio Mateus Assane³, Fabiana Pilarski⁴, Jonas Augusto Rizzato Paschoal⁵

Affiliations

¹ School of Pharmaceutical Sciences of Ribeirao Preto, Department of Biomolecular Sciences, University of Sao Paulo (USP), Ribeirao Preto, SP 14040-900, Brazil.
² Ecole de Biologie Industrielle, Ingénieurs Bioindustries, Paris, France.
³ Laboratory of Microbiology and Parasitology of Aquatic Organisms, Sao Paulo State University (Unesp), Aquaculture Center of Unesp, Jaboticabal, SP 14884-900, Brazil; Faculdade de Ciências Agrárias, Universidade Zambeze (UniZambeze), Ulónguè, Tete 0713-02, Mozambique.
⁴ Laboratory of Microbiology and Parasitology of Aquatic Organisms, Sao Paulo State University (Unesp), Aquaculture Center of Unesp, Jaboticabal, SP 14884-900, Brazil; Graduate Program in Agricultural and Livestock Microbiology, Sao Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, SP 14870-000, Brazil.
⁵ School of Pharmaceutical Sciences of Ribeirao Preto, Department of Biomolecular Sciences, University of Sao Paulo (USP), Ribeirao Preto, SP 14040-900, Brazil. Electronic address: paschoal@usp.br.

PMID: 36370561
DOI: 10.1016/j.foodchem.2022.134852

Abstract

An analytical method for the determination of erythromycin A (ERY) residues in fish fillet was developed, optimized, and validated employing a modified QuEChERS procedure associated to DLLME technique as a preconcentration step. The obtained LOD and the LOQ were 0.1 µg kg^-1 and 1 µg kg^-1, respectively. The validated method provides linearity in the range of 1 to 20 µg kg^-1, precision (CV < 6.3 %) and accuracy (recovery ranging from 103 to 110 %). The procedure was applied in an experimental study to evaluate the residual depletion profile of ERY in fish (Piaractus mesopotamicus) after oral administration. The treatment was carried out at a daily dose of 100 mg (kg BW)^-1 of ERY, for 7 consecutive days and with an average water temperature of 30 °C. A withdrawal time of 240°-day was estimated for eliminating ERY residues at concentration levels below the maximum residue limit considered (MRL 100 µg kg^-1).

Keywords: Aquaculture; DLLME; Erythromycin; Food safety; QuEChERS; Veterinary drugs residues.

MeSH terms

Administration, Oral
Animals
Characiformes*
Drug Residues* / analysis
Erythromycin

Substances

Erythromycin