SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe

Dorothea Anrather; Silvia Bagelova Polakova; Lubos Cipak; Juraj Gregan

doi:10.1007/978-1-0716-2863-8_2

SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe

Methods Mol Biol. 2023:2603:19-29. doi: 10.1007/978-1-0716-2863-8_2.

Authors

Dorothea Anrather¹, Silvia Bagelova Polakova^{2

3}, Lubos Cipak⁴, Juraj Gregan⁵

Affiliations

¹ Mass Spectrometry Facility, Max Perutz Labs, Vienna BioCenter (VBC), Vienna, Austria. dorothea.anrather@univie.ac.at.
² Institute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, Bratislava, Slovakia. silvia.bagelova@savba.sk.
³ Department of Genetics, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, Slovakia. silvia.bagelova@savba.sk.
⁴ Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia. lubos.cipak@savba.sk.
⁵ Advanced Microscopy Facility, VBCF and Department of Chromosome Biology, Max Perutz Labs, University of Vienna, Vienna BioCenter (VBC), Vienna, Austria. juraj.gregan@univie.ac.at.

PMID: 36370267
DOI: 10.1007/978-1-0716-2863-8_2

Abstract

Stable isotope labeling by amino acids in cell culture (SILAC) provides a powerful tool to quantify proteins and posttranslational modifications. Here we describe how to apply SILAC for protein identification and quantification in synchronous meiotic cultures induced by inactivation of the Pat1 kinase in the fission yeast Schizosaccharomyces pombe.

Keywords: Mass Spectrometry; Meiosis; Pat1; Proteome; SILAC; Schizosaccharomyces pombe; Yeast.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Meiosis
Proteins / metabolism
Proteomics
Schizosaccharomyces pombe Proteins* / genetics
Schizosaccharomyces pombe Proteins* / metabolism
Schizosaccharomyces* / metabolism

Substances

Proteins
Schizosaccharomyces pombe Proteins