Wheat bran fermented by Lactobacillus regulated the bacteria-fungi composition and reduced fecal heavy metals concentrations in growing pigs

Abstract

Lactobacillus fermentation can increase the value of wheat bran, but the benefits of fermented wheat bran for pig production are poorly understood. We evaluated the phenolic acid content of wheat bran fermented with Lactobacillus. The bacterial and fungal compositions, short-chain fatty acids, and heavy metals concentrations in the feces of growing pigs were determined, and the correlations between the bacterial and fungal compositions and short-chain fatty acid and heavy metals concentrations were also assessed. The concentrations of phenolic acids (caffeic acid, catechinic acid, and gallic acid) were higher in fermented bran than in control wheat bran. The diversity of feces bacterial species was significantly higher, whereas the diversity of fungi was lower in fermented wheat bran treatment than those in the control group, and pigs consuming fermented and control wheat bran with different bacterial and fungal compositions had different growth rates. The abundance of genera in fungi that were less abundant in the fermented group samples than in the control samples (including Wallemia, Trichosporon, Candida, Aspergillus, and unclassified_f__Microascaceae) was positively correlated with heavy metals concentrations in pig feces, and the abundances of these fungi were negatively correlated with caffeic acid, catechinic acid, and gallic acid concentrations. Metagenomic function predictions indicated that larger amounts of secondary metabolites were synthesized in the fermented group than in the control group. The results provide new insights into the roles of bacterial-fungal interactions in the growth and decreasing environmental pollution of pigs consuming fermented wheat bran.

Keywords: Bacteria–fungi composition; Growing pigs; Heavy metals; Lactobacillus strain; Metagenomic function; Wheat bran fermentation.