Genome-Wide Identification and Functional Analysis of the GASA Gene Family Responding to Multiple Stressors in Canavalia rosea

Genes (Basel). 2022 Oct 31;13(11):1988. doi: 10.3390/genes13111988.

Abstract

In plants, the Gibberellic Acid-Stimulated Arabidopsis (GASA) gene family is unique and responds to ubiquitous stress and hormones, playing important regulatory roles in the growth and development of plants, as well as in the resistance mechanisms to biotic and abiotic stress. In this study, a total of 23 CrGASAs were characterized in C. rosea using a genome-wide approach, and their phylogenetic relationships, gene structures, conserved motifs, chromosomal locations, gene duplications, and promoter regions were systematically analyzed. Expression profile analysis derived from transcriptome data showed that CrGASAs are expressed at higher levels in the flowers or fruit than in the leaves, vines, and roots. The expression of CrGASAs also showed habitat- and environmental-stress-regulated patterns in C. rosea analyzed by transcriptome and quantitative reverse transcription PCR (qRT-PCR). The heterologous induced expression of some CrGASAs in yeast enhanced the tolerance to H2O2, and some CrGASAs showed elevated heat tolerance and heavy metal (HM) Cd/Cu tolerance. These findings will provide an important foundation to elucidate the biological functions of CrGASA genes, especially their role in the ecological adaptation of specific plant species to tropical islands and reefs in C. rosea.

Keywords: Canavalia rosea; Gibberellic Acid-Stimulated Arabidopsis (GASA); ecological adaptability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis* / genetics
  • Arabidopsis* / metabolism
  • Canavalia / genetics
  • Canavalia / metabolism
  • Gene Expression Regulation, Plant
  • Hydrogen Peroxide / metabolism
  • Multigene Family
  • Phylogeny
  • Plant Proteins / metabolism

Substances

  • gibberellic acid
  • Plant Proteins
  • Hydrogen Peroxide

Grants and funding

This research was funded by the Guangdong Science and Technology Program (No. 2019B121201005), the Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences (No. ISEE2021ZD04), and the National Natural Sciences Foundation of China (No. U1701246 and 32270380). The funders had no roles in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.