Role of lncRNA Has2os in Skeletal Muscle Differentiation and Regeneration

Wanxin Chen; Weicai Chen; Peng Liu; Shiyu Qian; Shuang Tao; Mengchun Huang; Wanyi Xu; Cuiping Li; Xiaoyan Chen; Huizhu Lin; Zhenshu Qin; Jianxi Lu; Shujuan Xie

doi:10.3390/cells11213497

Role of lncRNA Has2os in Skeletal Muscle Differentiation and Regeneration

Cells. 2022 Nov 4;11(21):3497. doi: 10.3390/cells11213497.

Authors

Wanxin Chen¹, Weicai Chen¹, Peng Liu², Shiyu Qian³, Shuang Tao¹, Mengchun Huang¹, Wanyi Xu¹, Cuiping Li¹, Xiaoyan Chen¹, Huizhu Lin¹, Zhenshu Qin⁴, Jianxi Lu¹, Shujuan Xie^{1

5}

Affiliations

¹ Biotherapy Center, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, China.
² Laboratory Medicine, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, China.
³ Department of Public Health and Preventive Medicine, School of Medicine, Jinan University, Guangzhou 510632, China.
⁴ Department of Trauma Orthopaedics, Chenzhou First People's Hospital Affiliated to South China University, Chenzhou 423000, China.
⁵ Vaccine Research Institute of Sun Yat-Sen University, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, China.

Abstract

Long non-coding RNAs (lncRNAs) regulate a series of physiological processes and play an important role in development, metabolism and disease. Our previous studies showed that lncRNAs involved in skeletal muscle differentiation. Here, we demonstrated that lncRNA Has2os is highly expressed in skeletal muscle and significantly elevated during skeletal cell differentiation. The knockdown of Has2os inhibited myocyte fusion and impeded the expression of the myogenic factors MyHC and Mef2C. Mechanically, Has2os regulates skeletal muscle differentiation by inhibiting the JNK/MAPK signaling pathway. Furthermore, we also revealed that Has2os is involved in the early stage of regeneration after muscle injury, and the JNK/MAPK signaling pathway is activated at both protein and mRNA levels during early repair. Our results demonstrate the new function of lncRNA Has2os, which plays crucial roles during skeletal muscle differentiation and muscle regeneration, providing a basis for the therapy of lncRNA-related muscle diseases.

Keywords: Has2os; JNK/MAPK; lncRNA; regeneration; skeletal muscle differentiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / genetics
Muscle Development / genetics
Muscle, Skeletal / metabolism
Myoblasts / metabolism
RNA, Long Noncoding* / genetics
RNA, Long Noncoding* / metabolism

Substances

RNA, Long Noncoding

Grants and funding

This work was supported by grants from the Natural Science Foundation of Guangdong Province (2022A1515011413 and 2021A1515010477), the National Natural Science Foundation of China (31701116), the Guangzhou Science and Technology Plan Projects (202102020104), and the Guangdong Province Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation (2020B1212060018).