PRP8-Induced CircMaml2 Facilitates the Healing of the Intestinal Mucosa via Recruiting PTBP1 and Regulating Sec62

Yuequ Deng; Xiaoqing Xu; Fanze Meng; Jiaqi Lou; Yu Liao; Qi Li; Mengmeng Zhuang; Yong Sun

doi:10.3390/cells11213460

PRP8-Induced CircMaml2 Facilitates the Healing of the Intestinal Mucosa via Recruiting PTBP1 and Regulating Sec62

Cells. 2022 Nov 1;11(21):3460. doi: 10.3390/cells11213460.

Authors

Yuequ Deng^{1

2}, Xiaoqing Xu^{1

2}, Fanze Meng^{1

2}, Jiaqi Lou^{1

2}, Yu Liao^{1

2}, Qi Li^{1

2}, Mengmeng Zhuang^{1

2}, Yong Sun^{1

2}

Affiliations

¹ Department of Burn Surgery, The Affiliated Huaihai Hospital of Xuzhou Medical University, Xuzhou 221004, China.
² Department of Burn Surgery, The 71st Group Army Hospital of PLA, Xuzhou 221004, China.

Abstract

Background: Multiple organ dysfunction syndrome (MODS) occurs in the gastrointestinal tract and injured intestinal mucosa is the anatomical basis for various diseases. The expression of circular RNAs (circRNAs) is implicated in many diseases; however, the role of circRNAs in intestinal mucosal injury is yet to be discovered. Our preliminary gene microarray analysis revealed a novel circular RNA, circMaml2, with a significant intestinal mucosal protection effect. Its expression was found to decrease in severely burned intestinal mucosal tissue, whereas its overexpression might facilitate the reconstruction of the injured intestinal mucous membrane.

Methods: The function of circMaml2 in cell proliferation and migration was studied in MC38 cells. The repair function of circMaml2 was tested on the intestinal mucosa of mice. RNA-binding protein polypyrimidine tract-binding protein 1(PTBP1) was selected by pull-down assay and mass spectrometry (MS). RNA immunoprecipitation (RIP) was performed to confirm the binding of circMaml2 and PTBP1 and to study PTBP1 and its downstream target, early B-cell factor 1(Ebf1). Bioinformatics software forecast analysis and dual-luciferase reporter assay were performed to ascertain miR-683 and Sec62 as the downstream targets of circMaml2 and miR-683, respectively. Furthermore, PRP8 was discovered to promote the biogenesis of circMaml2.

Results: CircMaml2 promotes cell proliferation and migration of MC38 cells and the repair of the intestinal mucosa of mice. This effect is brought about by combining with PTBP1 to improve Ebf1 and interacting with miR-683 to regulate Sec2. Furthermore, PRP8 was discovered to promote the biogenesis of circMaml2.

Conclusions: This is the first reported study of the effect of circMaml2 on intestinal mucosal repair.

Keywords: PRP8; PTBP1; RNA-binding protein; burn; circMaml2; miR-683.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Heterogeneous-Nuclear Ribonucleoproteins / genetics
Heterogeneous-Nuclear Ribonucleoproteins / metabolism
Intestinal Mucosa / metabolism
Mice
MicroRNAs* / genetics
Polypyrimidine Tract-Binding Protein* / genetics
Polypyrimidine Tract-Binding Protein* / metabolism
RNA, Circular / genetics
Transcription Factors / metabolism

Substances

Polypyrimidine Tract-Binding Protein
Heterogeneous-Nuclear Ribonucleoproteins
RNA, Circular
Transcription Factors
MicroRNAs
Ptbp1 protein, mouse

Grants and funding

This work was supported by the National Natural Science Foundation of China (81772082), the Natural Science Foundation of Jiangsu Province (BK20211060), the Natural Science Foundation of Xuzhou (KC20085, KC21205), and the Postgraduate Innovation Program of Jiangsu Province (KYCX21_2677).