The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulfated Glycosaminoglycans

Yann D Ladner; Mauro Alini; Angela R Armiento

doi:10.1007/978-1-0716-2839-3_9

The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulfated Glycosaminoglycans

Methods Mol Biol. 2023:2598:115-121. doi: 10.1007/978-1-0716-2839-3_9.

Authors

Yann D Ladner^{1

2}, Mauro Alini³, Angela R Armiento⁴

Affiliations

¹ AO Research Institute Davos, Davos Platz, Switzerland.
² Institute for Biomechanics, ETH Zurich, Zurich, Switzerland.
³ AO Research Institute Davos, Davos Platz, Switzerland. mauro.alini@aofoundation.org.
⁴ UCB Pharma, Slough, UK.

PMID: 36355288
DOI: 10.1007/978-1-0716-2839-3_9

Abstract

The 1,9-dimethylmethylene blue (DMMB) assay enables the detection of sulfated glycosaminoglycans (sGAGs). This assay can be used to quickly quantify the sGAG content in a large number of samples using spectrophotometry. While this widespread assay appears straightforward, there are certain pitfalls that need to be considered.

Keywords: Cartilage; DMMB; Dimethylmethylene blue assay; Sulfated glycosaminoglycans; sGAG.

MeSH terms

Glycosaminoglycans*
Methylene Blue*
Spectrophotometry

Substances

A73025
dimethylmethylene blue
Glycosaminoglycans
Methylene Blue