Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation

Justyna M Buchert; Benedict Lotz; Solvig Diederichs; Wiltrud Richter

doi:10.1007/978-1-0716-2839-3_7

Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation

Methods Mol Biol. 2023:2598:75-85. doi: 10.1007/978-1-0716-2839-3_7.

Authors

Justyna M Buchert¹, Benedict Lotz², Solvig Diederichs¹, Wiltrud Richter³

Affiliations

¹ Research Centre for Experimental Orthopaedics, Heidelberg University Hospital, Heidelberg, Germany.
² Clinic for Orthopaedics and Trauma Surgery, Heidelberg University Hospital, Heidelberg, Germany.
³ Research Centre for Experimental Orthopaedics, Heidelberg University Hospital, Heidelberg, Germany. Wiltrud.Richter@med.uni-heidelberg.de.

PMID: 36355286
DOI: 10.1007/978-1-0716-2839-3_7

Abstract

Adipose-derived stromal cells (ASC) are a promising alternative cell source to chondrocytes as well as to bone marrow-derived mesenchymal stromal cells (BMSC) in cartilage tissue engineering and repair. Here we describe ASC isolation from liposuction by-products by collagenase-based tissue digestion combined with cell filtration and followed by monolayer attachment and expansion culture. Quality control requires confirmation of correct surface marker expression and multilineage differentiation potential by a trilineage differentiation assay.

Keywords: Adipose tissue; Adipose-derived stromal cells; Cartilage; Chondrogenesis; MSC.

MeSH terms

Adipose Tissue*
Bone Marrow Cells
Cartilage
Cell Differentiation
Cells, Cultured
Chondrocytes
Chondrogenesis*
Stromal Cells / metabolism