Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

Angela R Armiento; Yann D Ladner; Elena Della Bella; Martin J Stoddart

doi:10.1007/978-1-0716-2839-3_6

Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

Methods Mol Biol. 2023:2598:65-73. doi: 10.1007/978-1-0716-2839-3_6.

Authors

Angela R Armiento^{1

2}, Yann D Ladner^{1

3}, Elena Della Bella¹, Martin J Stoddart⁴

Affiliations

¹ AO Research Institute Davos, Davos Platz, Switzerland.
² UCB Pharma, Slough, UK.
³ Institute for Biomechanics, ETH Zürich, Zürich, Switzerland.
⁴ AO Research Institute Davos, Davos Platz, Switzerland. martin.stoddart@aofoundation.org.

PMID: 36355285
DOI: 10.1007/978-1-0716-2839-3_6

Abstract

Bone marrow-derived mesenchymal stromal cells (BM-MSC) are widely studied in the field of cartilage regeneration due to their capacity to differentiate into chondrocytes under specific in vitro culture conditions. This chapter describes the isolation of MSC from bone marrow aspirate, their expansion in monolayer, and the chondrogenic differentiation in pellet culture.

Keywords: Bone marrow; Chondrogenesis; MSC; Pellet culture; TGF-β1.

MeSH terms

Bone Marrow Cells
Bone Marrow*
Cell Differentiation
Cells, Cultured
Chondrocytes
Chondrogenesis
Humans
Mesenchymal Stem Cells*