Rapid and specific detection of Trichophyton rubrum and Trichophyton mentagrophytes using a loop-mediated isothermal amplification assay

Mi-Ran Seo; Hyo Seok Kim; Young Bok Lee; Sun Shin; Yeun-Jun Chung

doi:10.1016/j.mex.2022.101891

Rapid and specific detection of Trichophyton rubrum and Trichophyton mentagrophytes using a loop-mediated isothermal amplification assay

MethodsX. 2022 Oct 27:9:101891. doi: 10.1016/j.mex.2022.101891. eCollection 2022.

Authors

Mi-Ran Seo¹, Hyo Seok Kim¹, Young Bok Lee^{2

3}, Sun Shin^{4

5}, Yeun-Jun Chung^{3

4

5

6}

Affiliations

¹ ConnectaGen, Hanam 12918, Korea.
² Department of Dermatology, Uijeongbu St. Mary's Hospital, The Catholic University of Korea, Uijeongbu, Korea.
³ Department of Biomedicine & Health Sciences.
⁴ Department of Microbiology.
⁵ Precision Medicine Research Center.
⁶ IRCGP, The Catholic University of Korea, Seoul 06591, Republic of Korea.

Abstract

Trichophyton rubrum and Trichophyton mentagrophytes are the main causative pathogens of onychomycosis. However, the sensitivity and specificity of conventional microscopic tests are insufficient for reliable diagnoses of onychomycosis. In this study, we developed loop-mediated isothermal amplification (LAMP) assays for the rapid and specific identification of the two major Trichophytons spp. We designed LAMP primers targeting the internal transcribed spacer 1 region of the T. rubrum and T. mentagrophytes. Through rigorous optimization of the reaction conditions, we defined a universal reaction condition for both LAMP assays.

Keywords: Trichophyton mentagrophytes; Trichophyton rubrum; loop-mediated isothermal amplification; onychomycosis.