Enhanced cytotoxic effect of doxorubicin conjugated gold nanoparticles on breast cancer model

Amna H Faid; Samia A Shouman; Yehia A Badr; Marwa Sharaky

doi:10.1186/s13065-022-00889-9

Enhanced cytotoxic effect of doxorubicin conjugated gold nanoparticles on breast cancer model

BMC Chem. 2022 Nov 9;16(1):90. doi: 10.1186/s13065-022-00889-9.

Authors

Amna H Faid¹, Samia A Shouman², Yehia A Badr³, Marwa Sharaky²

Affiliations

¹ National Institute of Laser Enhanced Science, Cairo University, Giza, Egypt. amna.hussein@cu.edu.eg.
² National Cancer Institute (NCI), Cairo University, Cancer biology, Giza, Egypt.
³ National Institute of Laser Enhanced Science, Cairo University, Giza, Egypt.

Abstract

Background: The difficulty of achieving targeted drug delivery following administration of presently marketed anticancer therapeutics is still a concern. Metallic nanoparticles (NPs) appear to be promising in this regard. The present study focused on the use of gold nanoparticles (AuNPs) as a drug carrier for anticancer Doxorubicin (DOX) forming DOX-AuNPs nanocomposite. The anticancer effect of the prepared nanocomposite was evaluated using SRP essay on breast cancer cell line (MCF7) for different incubation times (24 h,48, and72hr). The prepared DOX-AuNPs nanocomposite was investigated by UV-visible spectroscopy, TEM, fluorescence spectroscopy, and FTIR spectroscopy.

Results: Our results showed that the prepared AuNPs and DOX-AuNPs nanocomposite have spherical and small size10 ± 2 nm and 12 ± 2 nm respectively. The potential cytotoxicity of the DOX-AuNPs nanocomposite on the MCF7 cell line was significantly increased compared to free DOX. The 20 µM DOX- AuNPs nanocomposite produced a similar decrease in cell survival as 80 µM free DOX.

Conclusion: Future work is in progress to investigate the positive effects of the prepared nanocomposite for chemo-photothermal combination treatment.

Keywords: Breast carcinoma cell line (MCF7); Cancer; Doxorubicin; Drug delivery; Gold nanoparticles.