Hymenobacter lucidus sp. nov., and Hymenobacter nitidus sp. nov., isolated from soil

Abstract

Two novel bacterial strains BT178^T and BT635^T were isolated from soil collected in Gyeonggi province, Republic of Korea. Both strains were Gram-negative, rod-shaped circular, and had convex, glistening, pink-colored colonies. Phylogenetic analysis based on 16S rRNA gene sequences showed that each of the strains BT178^T and BT635^T formed an individual lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia, phylum Bacteroidetes). The 16S rRNA gene sequence similarity between the two strains BT178^T and BT635^T were 97.7%. Both strains had Q-7 as the major respiratory quinone and phosphatidylethanolamine (PE) as the major polar lipids. The main cellular fatty acids of strain BT178^T were iso-C_15:0, anteiso-C_15:0, and those of strain BT635^T were iso-C_15:0 and Summed Feature 4 (iso-C_17:1 I / anteiso-C_17:1 B). Based on the result of polyphasic taxonomic analysis, two novel species, Hymenobacter lucidus BT178^T (= KCTC 72333^T = NBRC 115442^T) and Hymenobacter nitidus BT635^T (= KACC 21904^T = NBRC 115443^T) were proposed.

Keywords: Hymenobacter; Hymenobacteraceae; Taxonomy.