A Method to Monitor Activity of SARS-CoV-2 Nsp3 from Cells

Christopher Rainville; David E Sterner; Kumar Suresh

doi:10.1007/978-1-0716-2803-4_16

A Method to Monitor Activity of SARS-CoV-2 Nsp3 from Cells

Methods Mol Biol. 2023:2591:269-282. doi: 10.1007/978-1-0716-2803-4_16.

Authors

Christopher Rainville¹, David E Sterner¹, Kumar Suresh²

Affiliations

¹ Progenra Inc, Malvern, PA, USA.
² Progenra Inc, Malvern, PA, USA. kumar@progenra.com.

PMID: 36350554
DOI: 10.1007/978-1-0716-2803-4_16

Abstract

SARS-CoV-2 protease Nsp3 is a therapeutic target for developing anti-SARS-CoV-2 drugs. Nsp3 is a large multi-spanning membrane protein, and its characterization in vitro has been challenging. Here we describe an in vitro assay to characterize the biochemical activity of full-length Nsp3 isolated from cells. The assay can be used to evaluate Nsp3 inhibitors.

Keywords: Deubiquitinase; ISG15-CHOP2; Nsp3; PLpro; SARS-CoV-2; Ub-Rh110; Ubiquitin; deISGylase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

COVID-19*
Coronavirus 3C Proteases
Humans
SARS-CoV-2*
Ubiquitin / metabolism
Ubiquitins / metabolism

Substances

Coronavirus 3C Proteases
Ubiquitins
Ubiquitin