Zeolitic imidazolate frameworks as effective crystalline supports for aspergillus-based laccase immobilization for the biocatalytic degradation of carbamazepine

Mbongiseni Lungelo Dlamini; Mahadi Lesaoana; Izak Kotze; Heidi Lynn Richards

doi:10.1016/j.chemosphere.2022.137142

Zeolitic imidazolate frameworks as effective crystalline supports for aspergillus-based laccase immobilization for the biocatalytic degradation of carbamazepine

Chemosphere. 2023 Jan;311(Pt 2):137142. doi: 10.1016/j.chemosphere.2022.137142. Epub 2022 Nov 5.

Authors

Mbongiseni Lungelo Dlamini¹, Mahadi Lesaoana¹, Izak Kotze¹, Heidi Lynn Richards²

Affiliations

¹ Molecular Sciences Institute, School of Chemistry, University of the Witwatersrand, Private Bag X3, Johannesburg, 2050, South Africa.
² Molecular Sciences Institute, School of Chemistry, University of the Witwatersrand, Private Bag X3, Johannesburg, 2050, South Africa. Electronic address: heidi.richards@wits.ac.za.

PMID: 36347352
DOI: 10.1016/j.chemosphere.2022.137142

Abstract

In this study, zeolitic imidazolate frameworks (ZIF) were employed as effective porous supports for laccase enzyme attachment and further explored synergistic adsorption and biocatalytic degradation of carbamazepine (CBZ) in aqueous solutions. Characterization results from FTIR and NMR analysis confirmed successful incorporation of the laccase enzyme onto ZIF particles. Further analyses from SEM and TEM revealed rhombic dodecahedral morphologies of ZIF crystals with crusts of the enzyme observed on the particles' surface. The carbamazepine degradation results showed that immobilization of the laccase improved its stability and resistance at various pH's, in comparison to the free enzyme. The immobilized laccase also exhibited relatively higher activities across the studied temperature range compared to the free form. Kinetic studies revealed a negligible decline in velocity, Vmax after immobilization, evaluated to be 0.873 and 0.692 mg L^-1 h^-1 for the free and immobilized laccase, respectively. The immobilized laccase demonstrated improved stabilities towards organic solvents, which qualifies the composite's application in real wastewater samples. In which case, the laccase-ZIF composite proved effective in CBZ decontamination with an efficiency of ∼92%. Furthermore, the immobilized laccase exhibited appreciable storage stabilities (∼70% residual activity) for up to 15 days before any significant loss in activity.

Keywords: Biocatalyst; Biodegradation; Carbamazepine; Laccase; Zeolitic imidazolate frameworks.