Identification of novel bioactive proteins and their produced oligopeptides from Torreya grandis nuts using proteomic based prediction

Rabia Durrani; Yang Meiyun; Baoru Yang; Erwann Durand; André Delavault; Hou Bowen; Huan Weiwei; Long Yiyang; Song Lili; Gao Fei

doi:10.1016/j.foodchem.2022.134843

Identification of novel bioactive proteins and their produced oligopeptides from Torreya grandis nuts using proteomic based prediction

Food Chem. 2023 Mar 30;405(Pt A):134843. doi: 10.1016/j.foodchem.2022.134843. Epub 2022 Nov 3.

Authors

Rabia Durrani¹, Yang Meiyun², Baoru Yang³, Erwann Durand⁴, André Delavault⁵, Hou Bowen², Huan Weiwei⁶, Long Yiyang², Song Lili⁷, Gao Fei⁸

Affiliations

¹ State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'an, Zhejiang Province 311300, China.
² Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A&F University, Hangzhou 311300, China.
³ Food Chemistry and Food Development, Department of Life Technologies, University of Turku, University of Turku, Turku FI-20014, Finland.
⁴ CIRAD, UMR QUALISUD, Montpellier, France; Qualisud, Univ Montpellier, Avignon Université, CIRAD, Institut Agro, Université de la Réunion, Montpellier, France.
⁵ Karlsruhe Institute of Technology (KIT), Institute of Process Engineering in Life Sciences, Fritz-Haber-Weg 4, Building 30.43, 1(st) Floor, Room 104, Karlsruhe 76131, Germany.
⁶ Zhejiang Provincial Key Laboratory of Chemical Utilization of Forestry Biomass, College of Chemistry and Materials Engineering, Zhejiang A & F University, Hangzhou, Zhejiang 311300, China.
⁷ State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'an, Zhejiang Province 311300, China. Electronic address: lilisong@zafu.edu.cn.
⁸ Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A&F University, Hangzhou 311300, China. Electronic address: wanttofly407@qq.com.

PMID: 36347203
DOI: 10.1016/j.foodchem.2022.134843

Abstract

Torreya grandis nut is a chief functional food in China consumed for centuries. Besides its rich protein composition, increasing studies are now focusing on T. grandis functional proteins that have not yet identified. In this study, liquid chromatography coupled with mass spectrometry detection of smaller and major proteins, revealed that the major peptide was 36935.00 Da. Proteome sequencing annotated 142 proteins in total. Bioactive proteins such as defensin 4 was annotated and its anti-microbial function was verified. Finally, functional oligopeptides were predicted by searching sequences of digested peptides in databases. Ten group of oligopeptides were suggested to exhibit antioxidant, Angiotensin-converting enzyme inhibition, anti-inflammatory. The predicted antioxidant activity was experimentally validated. It is interesting that a peptide GYCVSDNN digested from defensin 4 showed antioxidant activity. This study reports novel functional peptides from T. grandis nuts that have not been isolated and/or included as functional ingredients in nutraceuticals and in food industry.

Keywords: Angiotensin-converting enzyme inhibiting; Bioactive peptide; Functional proteins; Proteome; Torreya grandis nuts.

MeSH terms

Antioxidants / analysis
Defensins / analysis
Nuts* / chemistry
Oligopeptides / analysis
Peptides / analysis
Proteomics
Taxaceae* / chemistry

Substances

Antioxidants
Oligopeptides
Peptides
Defensins