Nitrogen fertilizer amount has minimal effect on rhizosphere bacterial diversity during different growth stages of peanut

Zheng Yang; Lin Li; Wenjuan Zhu; Siyuan Xiao; Siyu Chen; Jing Liu; Qian Xu; Feng Guo; Shile Lan

doi:10.7717/peerj.13962

Nitrogen fertilizer amount has minimal effect on rhizosphere bacterial diversity during different growth stages of peanut

PeerJ. 2022 Nov 2:10:e13962. doi: 10.7717/peerj.13962. eCollection 2022.

Authors

Zheng Yang¹, Lin Li², Wenjuan Zhu¹, Siyuan Xiao¹, Siyu Chen¹, Jing Liu¹, Qian Xu¹, Feng Guo³, Shile Lan¹

Affiliations

¹ College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, China.
² College of Agronomy, Hunan Agricultural University, Changsha, Hunan, China.
³ Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.

Abstract

The impact of short-term nitrogen fertilizer input on the structure and diversity of peanut rhizosphere microbiota (RM) at different growth stages (GSs) was explored in the southern paddy soil planting environment. Three levels of nitrogen were applied in the field: control (LN, 0 kg/hm²), medium nitrogen (MN, 55.68 kg/hm²), and high nitrogen (HN, 111.36 kg/hm²). The rhizosphere soil was collected during four GSs for high-throughput sequencing and chemical properties analysis. The effect of nitrogen fertilizer application on peanut RM was minimal and was obvious only at the seedling stage. In the four peanut GSs, a significant increase in relative abundance was observed for only one operational taxonomic unit (OTU) of Nitrospira under HN conditions at the seedling stage and mature stage, while there was no consistent change in other OTUs. The difference in RM among different peanut GSs was greater than that caused by the amount of nitrogen fertilizer. This may be due to the substantial differences in soil chemical properties (especially alkali-hydrolyzable nitrogen, pH, and available potassium or total potassium) among peanut GSs, as these significantly affected the RM structure. These results are of great value to facilitate deeper understanding of the effect of nitrogen fertilizer on peanut RM structure.

Keywords: Arachis hypogaea; High-throughput sequencing; Nitrogen fertilizer; Peanut; Rhizosphere microbiota.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arachis*
Bacteria
Fertilizers* / analysis
Nitrogen / pharmacology
Potassium / analysis
Rhizosphere
Seedlings / chemistry
Soil / chemistry
Soil Microbiology

Substances

Fertilizers
Nitrogen
Soil
Potassium

Grants and funding

This research was funded by the National Key R & D Program of China, grant number 2018YFD1000902. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.