PGLN: A newly identified amino phosphoglycolipid species in Thermus thermophilus HB8

Naoki Nemoto; Masahiko Kawaguchi; Kei Yura; Haruo Shimada; Yoshitaka Bessho

doi:10.1016/j.bbrep.2022.101377

PGLN: A newly identified amino phosphoglycolipid species in Thermus thermophilus HB8

Biochem Biophys Rep. 2022 Nov 2:32:101377. doi: 10.1016/j.bbrep.2022.101377. eCollection 2022 Dec.

Authors

Naoki Nemoto¹, Masahiko Kawaguchi¹, Kei Yura^{2

3

4}, Haruo Shimada^{5

6}, Yoshitaka Bessho^{3

7

8}

Affiliations

¹ Faculty of Advanced Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba, 275-0016, Japan.
² Graduate School of Humanities and Sciences, Ochanomizu University, 2-1-1 Otsuka, Bunkyo, Tokyo, 112-8610, Japan.
³ Center for Interdisciplinary AI and Data Science, Ochanomizu University, 2-1-1 Otsuka, Bunkyo, Tokyo, 112-8610, Japan.
⁴ Graduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku, Tokyo, 169-8555, Japan.
⁵ BioChromato, Inc, 1-12-19 Honcho, Fujisawa, Kanagawa, 251-0053, Japan.
⁶ School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan.
⁷ Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo, 113-8657, Japan.
⁸ RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo, 679-5148, Japan.

Abstract

Thermus thermophilus has several minor lipid molecules with structures that have not been described yet. In this study, we identified a new lipid molecule in T. thermophilus HB8 with an amino group at the polar head, by detecting lipid spots with HPTLC and mass spectrometry. The structure of the lipid resembles an amino sugar phospholipid, except for the glucosamine that lacks an acetyl group. We named this amino phosphoglycolipid PGLN, and proposed its synthetic pathway from a precursor, phosphatidyl-glyceric alkylamine. The primary amine structure of PGLN may contribute to high temperature adaptation through electrostatic interactions between the head groups.

Keywords: Amino phospholipid; Glucosamine; Glycolipid; Phospholipid synthetic pathway; Polarlipid; Thermophiles.