Expression patterns and biological function of BCL2L10 during mouse preimplantation development

Yong Liu; Jing Xin; Shengnan Zhang; Qingmei Li; Wenying Wang; Ji Chen; Xin Ming; Xiaoqing Wu; Xinyan Cao; Wei Cui; Hongcheng Wang; Wenyong Li

doi:10.1016/j.gep.2022.119285

Expression patterns and biological function of BCL2L10 during mouse preimplantation development

Gene Expr Patterns. 2022 Dec:46:119285. doi: 10.1016/j.gep.2022.119285. Epub 2022 Oct 29.

Authors

Yong Liu¹, Jing Xin², Shengnan Zhang², Qingmei Li², Wenying Wang², Ji Chen², Xin Ming², Xiaoqing Wu², Xinyan Cao³, Wei Cui⁴, Hongcheng Wang⁵, Wenyong Li⁶

Affiliations

¹ Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, College of Biological and Food Engineering, Fuyang Normal University, Fuyang, 236037, China; Department of Veterinary and Animal Sciences, Animal Models Core Facility, Institute for Applied Life Sciences (IALS), University of Massachusetts Amherst, Amherst, MA, 01002, United States.
² Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, College of Biological and Food Engineering, Fuyang Normal University, Fuyang, 236037, China.
³ Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, No.4899 Juye Street, Jingyue District, Changchun, 130112, China.
⁴ Department of Veterinary and Animal Sciences, Animal Models Core Facility, Institute for Applied Life Sciences (IALS), University of Massachusetts Amherst, Amherst, MA, 01002, United States.
⁵ Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, College of Biological and Food Engineering, Fuyang Normal University, Fuyang, 236037, China; Linquan Modern Agricultural Technology Cooperation and Extension Service Center, The Anhui Agricultural University's Comprehensive Experimental Station in the Northwest of Anhui Province, Linquan, Anhui, 236400, China. Electronic address: wanghc@fynu.edu.cn.
⁶ Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, College of Biological and Food Engineering, Fuyang Normal University, Fuyang, 236037, China. Electronic address: liwenyong@fynu.edu.cn.

PMID: 36341977
DOI: 10.1016/j.gep.2022.119285

Abstract

BCL2-like 10 (BCL2L10) is abundantly expressed in mammalian oocytes and plays a crucial role in the completion of oocyte meiosis. However, the expression patterns of BCL2L10 and its biological functions during preimplantation development have not been well characterized. Here, we investigated the spatiotemporal expressions of Bcl2l10 during mouse preimplantation development using RT-qPCR and immunofluorescence and its biological function using siRNA and morpholino injection into pronuclear embryos. Results from RT-qPCR showed that Bcl2l10 was highly expressed in the metaphase Ⅱ-stage oocytes and pronuclear-stage embryos, but expression markedly decreased from the two-cell stage onwards and was no longer detected at the four-cell stage and beyond. Immunofluorescence staining showed that BCL2L10 was detectable throughout preimplantation development and localized in the cytoplasm and nuclei. Knocking down Bcl2l10 resulted in a reduced blastocyst formation rate (P < 0.01) and decreased expression of OCT4, NANOG, and SOX17 (P < 0.05). We concluded that the role of BCL2L10 is strongly associated with developmental competence of preimplantation mouse embryos.

Keywords: BCL2L10; Developmental competence; Expression patterns; Mouse embryo; Preimplantation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blastocyst / metabolism
Cytoplasm
Embryonic Development* / genetics
Gene Expression Regulation, Developmental
Mammals
Mice
Oocytes* / metabolism