The purpose of this retrospective study was to describe antimicrobial resistance trends in Klebsiella isolates cultured from milk samples submitted to the Wisconsin Veterinary Diagnostic Laboratory for bovine mastitis testing. In vitro antimicrobial susceptibility testing was conducted in 483 Klebsiella isolates cultured from 63,841 milk samples submitted from January 1, 2008, through December 31, 2019. The Wisconsin Veterinary Diagnostic Laboratory conducted antimicrobial susceptibility testing according to the Clinical and Laboratory Standards Institute by using a broth microdilution panel. Ten antimicrobials were tested in the panel: ampicillin, penicillin, erythromycin, oxacillin + 2% NaCl, pirlimycin, penicillin/novobiocin, tetracycline, ceftiofur, cephalothin, and sulfadimethoxine. Isolates were considered resistant to specific antimicrobials based on minimum inhibitory concentrations described in Clinical and Laboratory Standards Institute guidelines. No guidelines were available for sulfadimethoxine; isolates were categorized as resistant when bacterial growth was not inhibited. The proportion of isolates resistant to ceftiofur, cephalothin, or tetracycline did not increase over time. For sulfadimethoxine, the proportion of resistant isolates decreased over time. These results do not demonstrate a trend toward increasing antimicrobial resistance among Klebsiella isolates. Despite that, antimicrobial resistance should continue to be monitored.
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