Characterization of prickle isoform-specific pk pk1 and pk sple1 mutations in Drosophila melanogaster

Abstract

We used paired-end next generation sequencing (NGS) to characterize the classic isoform-specific pk ^pk1 and pk ^sple1 mutations of the prickle gene in Drosophila melanogaster . Here we provide evidence that these previously reported null mutations are caused by either a tirant transposon insertion into the 5' UTR of pk ^pk1 or a premature stop codon in the second exon of pk ^sple1 . Additional likely benign missense mutations were identified in both mutant isoforms.