PMo12 as a redox mediator for bio-reduction of Cr(VI): Promotor or inhibitor?

Abstract

Slow reduction rate and low reduction ability were the main limitations of bio-reduction of Cr(VI). As an efficient redox mediator, how phosphomolybdic acid (PMo₁₂) affected bio-reduction of Cr(VI) was worthy of exploration. In this study, short-term and long-term effects of PMo₁₂ on Cr(VI) reduction were investigated to reveal the relevant mechanism. After evaluating the short-term effect of PMo₁₂ concentration from 0.05 to 1.00 mM on Cr(VI) bio-reduction, 0.50 mM was found to be optimum by improving Cr(VI) reduction rate by 16.3 % and microbial electron transport system activity (ETSA) by 43.0 % with Cr(VI) reduction efficiency of 100 % in short-term (22 h) batch experiments. By contrast, in long-term (28 days) continuous flow experiments, 0.50 mM PMo₁₂ exhibited serious inhibition on Cr(VI) bio-reduction. The cumulative toxicity of Mo, strong oxidative stress (reactive oxygen species increased by 16.5 %), the inhibition of extracellular polymeric substances production and the reduction of microbial activity were proved to be the main inhibition mechanism. In terms of microbial electron transport system, the main electron carriers including flavin mononucleotide (FMN), nitrate reductase (NAR), nitrite reductase (NIR) were seriously inhibited. BugBase analysis confirmed that the relative abundance of biofilm forming bacteria decreased after PMo₁₂ addition, and the relative abundance of oxidative stress tolerance bacteria continued to increase.

Keywords: Cr(VI) bio-reduction; Inhibition; Phosphomolybdic acid (PMo(12)); Promotion.