Anti-hyperlipidemia on rats in vivo and new compounds from the seeds of Psoralea corylifolia

Abstract

In this study, it was confirmed at first time that the crude extracts of Psoralea corylifolia seeds (PCE34) can reduce serum lipids (AST, ALT, TG, TC, LDL, ALP, ACP and LDH), body weight and serum sugar, increase HDL and serum insulin in hyperlipidemic wistar rat induced by high-fat diet in vivo. Furthermore, eight new chalcones 1-8, one new flavanone 12, one new coumarin 14, three new meroterpenes 15-17 and one new bakuchiol 20 together with seven known compounds (9-11, 13, 18-19 and 21) were isolated from the PCE34. Their structures were elucidated based on analyses of their spectroscopic (UV, CD, NMR and HREIMS) data. All the isolates were evaluated for in vitro inhibitory activity against DGAT1/2, PTP1B and α-Glucosidase. Among them, compounds 1-3, 8-11, 14-17, 19 and 20 were found to exhibit selective inhibitory activity on DGAT1 with IC₅₀ values ranging from 66.7 ± 1.2 to 87.2 ± 1.3 μM; 1, 8-12, 14 and 20 has the best inhibit active on PTP1B with IC₅₀ values ranging from 13.8 ± 1.1 to 19.1 ± 1.6 μM; 1-12 and 14 displayed the significant inhibitory activities on α-Glucosidase with IC₅₀ values ranging from 29.1 ± 1.2 to 79.4 ± 1.2 μM.

Keywords: DGAT; Hyperlipidemia; PTP1B; Psoralea corylifolia; α-Glucosidase.