Effect of phosphate on cadmium immobilized by microbial-induced carbonate precipitation: Mobilization or immobilization?

Abstract

Microbial-induced carbonate precipitation (MICP) is a promising technology to immobilize/remediate heavy metals (HMs) like cadmium (Cd). However, the long-term stability of MICP-immobilized HMs is unclear, especially in farmland where chemical fertilization is necessary. Therefore, we performed MICP treatment on soils contaminated with various Cd compounds (CdCO₃, CdS, and CdCl₂) and added diammonium phosphate (DAP) to explore the impact of phosphate on the MICP-immobilized Cd. The results showed that MICP treatment was practical to immobilize the exchangeable Cd but to mobilize the carbonate and Fe/Mn oxide-bound Cd. After applying DAP, soil pH declined due to ammonium nitrification. At high P/Ca molar ratios (1/2 and 1), partial previously immobilized Cd was released due to the carbonate dissolution. Contrarily, exchangeable Cd transformed to less mobilizable Fe/Mn oxide-bound at low P/Ca molar ratios (1/4 and 1/8). Meanwhile, other treatments were also helpful in avoiding the release of immobilized Cd, such as applying non-ammonium phosphate and adding lime material after soil acidification. Our investigation suggested that the long-term stability of HMs in remediated sites should be carefully evaluated, especially in agricultural areas with phosphate and nitrogen fertilizer input.

Keywords: Cadmium; Immobilization; Microbial-induced carbonate precipitation (MICP); Phosphate; Soil acidification.