Flavones interact with fiber to affect fecal bacterial communities in vitro

Abstract

This study investigated the effects of the sugarcane flavones diosmin, diosmetin, luteolin, and tricin, and their interactions with sugarcane fiber on the modulation of gut microbiota using in vitro batch fermentation. The alteration of fecal fermentation bacterial profile was analyzed using 16S rRNA sequencing data, while the bioavailability of fiber was indicated by short-chain fatty acid (SCFA) production and metabolism of polyphenols was measured directly by phenolic metabolites. Application of diosmin, diosmetin, luteolin, and tricin without fiber had no significant effect on the overall microbiota profile after 24 h of fermentation. When fiber alone was added, total SCFA production increased, specifically that of propionic and valeric acids. However, when flavones were combined with fiber, synergistic effects on the modulation of relative abundances of different bacterial taxa was noted. In particular, the proportion of Prevotella spp. was significantly increased by the combinations of diosmin, luteolin, and tricin with fiber.

Keywords: 3-(3,4-dihydroxyphenyl)-propionic acid (PubChem CID: 348154); 3-(3-hydroxyphenyl)-propanoic acid (PubChem CID: 91); Acetic acid (PubChem CID: 176); Butyric acid (PubChem CID: 264); Diosmetin; Diosmetin (PubChem CID: 5281612); Diosmin; Diosmin (PubChem CID: 5281613); Flavones; In vitro batch fermentation; Isobuytric acid (PubChem CID: 6590); Isovaleric acid (PubChem CID: 10430); Luteolin; Luteolin (PubChem CID: 5280445); Microbiota; Propionic acid (PubChem CID: 1032); Sugarcane fiber; Tricin; Tricin (PubChem CID: 5281702); Valeric acid (PubChem CID: 7991).