Effects of palm olein and palm stearin on cecal and fecal microbiota of C57BL/6J mice under low and high fat intakes

Liting Wan; Lin Li; Xia Zhang; Bing Li; Janette M Harro; Mark E Shirtliff

doi:10.1016/j.foodchem.2022.134693

Effects of palm olein and palm stearin on cecal and fecal microbiota of C57BL/6J mice under low and high fat intakes

Food Chem. 2023 Mar 15;404(Pt B):134693. doi: 10.1016/j.foodchem.2022.134693. Epub 2022 Oct 19.

Authors

Liting Wan¹, Lin Li², Xia Zhang³, Bing Li⁴, Janette M Harro⁵, Mark E Shirtliff⁶

Affiliations

¹ School of Food Science and Technology, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Plant Protein Deep Processing, Ministry of Education, South China University of Technology, Guangzhou 510640, China; Department of Microbial Pathogenesis, School of Dentistry, University of Maryland - Baltimore, Baltimore, MD 21201, USA; School of Food Science and Engineering, Hainan University, Haikou 570228, China.
² School of Chemical Engineering and Energy Technology, Dongguan University of Technology, Dongguan 523808, China.
³ School of Food Science and Technology, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Plant Protein Deep Processing, Ministry of Education, South China University of Technology, Guangzhou 510640, China.
⁴ School of Food Science and Technology, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Plant Protein Deep Processing, Ministry of Education, South China University of Technology, Guangzhou 510640, China. Electronic address: bli@scut.edu.cn.
⁵ Department of Microbial Pathogenesis, School of Dentistry, University of Maryland - Baltimore, Baltimore, MD 21201, USA. Electronic address: JHarro@umaryland.edu.
⁶ Department of Microbial Pathogenesis, School of Dentistry, University of Maryland - Baltimore, Baltimore, MD 21201, USA; Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, Baltimore, MD 21201, USA.

PMID: 36323033
DOI: 10.1016/j.foodchem.2022.134693

Abstract

This work aimed to study the effects of dietary lipid composition and content on cecal and fecal microbiota of mice fed the following diets for 8 weeks: palm olein (PO)-based low-fat diet, PO-based high-fat diet, palm stearin (PS)-based low-fat diet, and PS-based high-fat diet. Increasing the dietary PS level favored the growth of Firmicutes over Bacteroidetes in the cecum and feces. In addition, it significantly elevated the total lipid (p < 0.01) and bile acid content (p < 0.01) in feces, resulting in the enrichment of fat-degrading and bile-acid tolerant genera within the families Ruminococcaceae and Lachnospiraceae. Although increasing the PO intake also caused obesity in mice, it did not affect the microbial structure. When fat intake is constant, only at a high-fat level can PS (vs PO) induce the above-mentioned microbial shifts. These results highlighted the combined roles of lipid quality and quantity on the gut microbiota.

Keywords: Bile acid; Dietary lipids; Gut microbiota; Lipid composition; Lipid content.

MeSH terms

Animals
Bile Acids and Salts
Cecum
Diet, High-Fat / adverse effects
Dietary Fats* / pharmacology
Feces
Mice
Mice, Inbred C57BL
Microbiota*
Palm Oil

Substances

Dietary Fats
Bile Acids and Salts
Palm Oil