Reprisal of Schima superba to Mn stress and exploration of its defense mechanism through transcriptomic analysis

Fiza Liaquat; Muhammad Farooq Hussain Munis; Samiah Arif; Muhammad Aamir Manzoor; Urooj Haroon; Iftikhar Hussain Shah; Muhammad Ashraf; Hyun Seok Kim; Shengquan Che; Liu Qunlu

doi:10.3389/fpls.2022.1022686

Reprisal of Schima superba to Mn stress and exploration of its defense mechanism through transcriptomic analysis

Front Plant Sci. 2022 Oct 6:13:1022686. doi: 10.3389/fpls.2022.1022686. eCollection 2022.

Authors

Fiza Liaquat^{1

2

3}, Muhammad Farooq Hussain Munis⁴, Samiah Arif¹, Muhammad Aamir Manzoor¹, Urooj Haroon⁴, Iftikhar Hussain Shah¹, Muhammad Ashraf⁵, Hyun Seok Kim^{2

3

6

7}, Shengquan Che⁸, Liu Qunlu⁸

Affiliations

¹ School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
² Department of Agriculture, Forestry, and Bioresources, Seoul National University, Seoul, South Korea.
³ Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, South Korea.
⁴ Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
⁵ National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
⁶ Interdisciplinary Program in Agricultural and Forest Meteorology, Seoul National University, Seoul, South Korea.
⁷ National Center for AgroMeteorology, Seoul, South Korea.
⁸ Department of Landscape Architecture, School of Design, Shanghai Jiao Tong University, Shanghai, China.

Abstract

One of the most diverse protein families, ATP-binding cassette (ABC) transporters, play a role in disease resistance, heavy metal tolerance, and food absorption.Differentially expressed genes contribute in the investigation of plant defense mechanisms under varying stress conditions. To elucidate the molecular mechanisms involved in Mn metal stress, we performed a transcriptomic analysis to explore the differential gene expression in Schima superba with the comparison of control. A total of 79.84 G clean data was generated and 6558 DEGs were identified in response to Mn metal stress. Differentially expressed genes were found to be involved in defense, signaling pathways, oxidative burst, transcription factors and stress responses. Genes important in metal transport were more expressive in Mn stress than control plants. The investigation of cis-acting regions in the ABC family indicated that these genes might be targeted by a large variety of trans-acting elements to control a variety of stress circumstances. Moreover, genes involved in defense responses, the mitogen-activated protein kinase (MAPK) signaling and signal transduction in S. superba were highly induced in Mn stress. Twenty ABC transporters were variably expressed on 1st, 5th, and 10th day of Mn treatment, according to the qRT PCR data. Inclusively, our findings provide an indispensable foundation for an advanced understanding of the metal resistance mechanisms. Our study will enrich the sequence information of S. superba in a public database and would provide a new understanding of the molecular mechanisms of heavy metal tolerance and detoxification.

Keywords: ATP Binding Cassette transporter; Manganese; Schima superba; hyperccumulater; transcriptome.