Shape-specific MOF-derived Cu@Fe-NC with morphology-driven catalytic activity: Mimicking peroxidase for the fluorescent- colorimetric immunosignage of ochratoxin

Mengting Chen; Xiaomeng Huang; Yuxuan Chen; Yiran Cao; Shengsen Zhang; Hongtao Lei; Weipeng Liu; Yingju Liu

doi:10.1016/j.jhazmat.2022.130233

Shape-specific MOF-derived Cu@Fe-NC with morphology-driven catalytic activity: Mimicking peroxidase for the fluorescent- colorimetric immunosignage of ochratoxin

J Hazard Mater. 2023 Feb 5;443(Pt A):130233. doi: 10.1016/j.jhazmat.2022.130233. Epub 2022 Oct 21.

Authors

Mengting Chen¹, Xiaomeng Huang², Yuxuan Chen², Yiran Cao², Shengsen Zhang², Hongtao Lei³, Weipeng Liu⁴, Yingju Liu⁵

Affiliations

¹ Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China; The Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
² Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.
³ The Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
⁴ Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China. Electronic address: weipeng_liu@scau.edu.cn.
⁵ Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Agricultural & Rural Pollution Abatement and Environmental Safety, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China. Electronic address: liuyingju@hotmail.com.

PMID: 36308933
DOI: 10.1016/j.jhazmat.2022.130233

Abstract

Ochratoxin A (OTA), which has strong hepatotoxicity and nephrotoxicity, can accumulate in the human body through the food chain; thus, the selective and effective detection of OTA is urgently required for food security. Nanozymes with hyperfine size and shape control have attracted attention because of their controllable structure and intrinsic activity. Herein, CuFe-bimetal coordinated N-doped carbon (Cu@Fe-NC) with morphology-driven peroxidase-mimicking activity was synthesized using Cu₂O with specific polygonal cubes and fully exposed {111} crystalline facets as the template to produce a CuFe-bimetallic metal organic framework (MOF) and further treating CuFe-MOF with high-temperature pyrolysis. N-doping can confer electronegativity to exhibit high affinity, while the large surface area of the porous carbon support can facilitate rapid adsorption-desorption equilibrium. Using the peroxidase-mimicking Cu@Fe_0.5-NC as a carrier, a versatile immunoassay for the detection of OTA was implemented based on the ratiometric fluorescence and the localized surface plasmon resonance peak shift, achieving a detection limit of 0.52 ng/L in the range of 0.001-10 μg/L. Therefore, the strategy of enhancing enzyme-mimicking activity using specific shapes and crystalline facets may open new avenues for food and environmental analysis.

Keywords: Immunosensor; Interface engineering; Morphology-driven; Ochratoxin A; Peroxidase-mimicking.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbon / chemistry
Colorimetry
Coloring Agents
Humans
Metal-Organic Frameworks* / chemistry
Ochratoxins*
Oxidoreductases
Peroxidase
Peroxidases / chemistry

Substances

Metal-Organic Frameworks
Peroxidase
Ochratoxins
Peroxidases
Oxidoreductases
Carbon
Coloring Agents