Adaptation to low nitrogen and salt stresses in the desert poplar by effective regulation of nitrogen assimilation and ion balance

Abstract

As a main desert plant from arid regions of Central Asia, Populus euphratica always encounters with nitrogen shortage in its long life, apart from salt or drought stress. However, it remains unknown how this species responds to low nitrogen and combined stresses of low nitrogen and salinity. Thus, saplings of P. euphratica with uniform size were exposed to normal or low nitrogen condition (150 and 15 ppm ammonium nitrate separately) individually or in combination with salinity. Under low nitrogen conditions we found a positive effect on P. euphratica root growth, which could be associated to high level of nitrogen allocation to support root growth and effective regulation of nitrogen assimilation in comparison with the other poplar species reported before. Under salt stress the root growth of P. euphratica was significantly inhibited, with the side effects of oxidative stress, as saplings stored higher Na⁺ and Cl^- contents in roots. Under the combined stressors of both salinity and low nitrogen, P. euphratica undergo a risky strategy, as stimulated root growth is accompanied by further oxidative stress.The concentrations of root K⁺ and whole plant NO₃^- were increased to support the tolerance of the combined stressors in P. euphratica, showing same characteristics with halophytes. Overall, our results provide evidence that the desert poplar can adapt to the salt stress/low nitrogen bundle, by effective regulation of nitrogen assimilation and ion homoeostasis.

Keywords: Ion balance; Low nitrogen under salinity; Nitrate reductase; Oxidative stress; Root growth; Sodium distribution.