Screening Metagenomes for Algae Cell Wall Carbohydrates Degrading Hydrolases in Enrichment Cultures

Methods Mol Biol. 2023:2555:125-137. doi: 10.1007/978-1-0716-2795-2_9.

Abstract

Sustainable use of natural products is one of the key challenges for the future. An increasing focus is on marine organic matter, mostly algae. New biotechnological tools for processing high amounts of micro- and macroalgae are necessary for efficient industrial degradation of marine matter. Secreted glycosyl hydrolases can be enriched and tested on the specific algae cell wall polymers of all algae groups (Rhodophyta; Phaeophyceae; Chlorophyta/Charophyta). Metagenomic analyses established new possibilities to screen algae-associated microbiomes for novel degrading enzymes in combination with sequence-based function prediction.

Keywords: Algae; Biotechnology; Enrichment culture; Enzymatic degradation; Glycoside hydrolases; Meta-omics; Sustainability.

MeSH terms

  • Carbohydrates
  • Cell Wall
  • Hydrolases / metabolism
  • Metagenome*
  • Rhodophyta* / metabolism

Substances

  • Hydrolases
  • Carbohydrates