Detection of apoptosis by [18F]ML-10 after cardiac ischemia-reperfusion injury in mice

Abstract

Objective: Myocardial infarction leads to ischemic heart disease and cell death, which is still a major obstacle in western society. In vivo imaging of apoptosis, a defined cascade of cell death, could identify myocardial tissue at risk.

Methods: Using 2-(5-[¹⁸F]fluoropentyl)-2-methyl-malonic acid ([¹⁸F]ML-10) in autoradiography and positron emission tomography (PET) visualized apoptosis in a mouse model of transient ligation of the left anterior descending (LAD) artery. 2-deoxy-2-[18F]fluoro-D-glucose ([¹⁸F]FDG) PET imaging indicated the defect area. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) histology stain indicated cardiac apoptosis.

Results: [¹⁸F]ML-10 uptake was evident in the ischemic area after transient LAD ligation in ex vivo autoradiography and in vivo PET imaging. Detection of [¹⁸F]ML-10 is in line with the defect visualized by [¹⁸F]FDG and the histological approach of TUNEL staining.

Conclusion: The tracer [¹⁸F]ML-10 is suitable for detecting apoptosis after transient LAD ligation in mice.

Keywords: Apoptosis; Autoradiography; Cardiac ischemia–reperfusion injury; Positron emission tomography; [18F]FDG; [18F]ML-10.