Integrative transcriptome analysis revealed the pathogenic molecular basis of Rhizoctonia solani AG-3 TB at three progressive stages of infection

Xinchun Li; Mengnan An; Chuantao Xu; Lianqiang Jiang; Fangfang Yan; Yang Yang; Chong Zhang; Yuanhua Wu

doi:10.3389/fmicb.2022.1001327

Integrative transcriptome analysis revealed the pathogenic molecular basis of Rhizoctonia solani AG-3 TB at three progressive stages of infection

Front Microbiol. 2022 Oct 11:13:1001327. doi: 10.3389/fmicb.2022.1001327. eCollection 2022.

Authors

Xinchun Li¹, Mengnan An¹, Chuantao Xu^{1

2}, Lianqiang Jiang³, Fangfang Yan⁴, Yang Yang⁵, Chong Zhang¹, Yuanhua Wu¹

Affiliations

¹ Liaoning Key Laboratory of Plant Pathology, College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
² Luzhou Branch of Sichuan Province Tobacco Company, Luzhou, China.
³ Liangshan Branch of Sichuan Province Tobacco Company, Xichang, China.
⁴ Panzhihua Branch of Sichuan Province Tobacco Company, Panzhihua, China.
⁵ Yibin Branch of Sichuan Province Tobacco Company, Yibin, China.

Abstract

Rhizoctonia solani has a broad host range and results in significant losses in agricultural production. Here, an integrated transcriptomic analysis was performed to reveal the critical genes responsible for the pathogenesis of R. solani AG-3 TB on Nicotiana tabacum at different infection stages. The results showed that various differential expressed genes (DEGs) were enriched in fatty acid metabolism, amino sugar, carbon metabolism, and cellular carbohydrate biosynthetic process at the early (6-12 hpi), middle (24-36 hpi), and late stage (48-72 hpi) of infection. Specifically, several critical genes such as shikimate kinase that were involved in the biosynthesis of an important fungal toxin, phenylacetic acid (PAA) showed markedly increase at 24 hpi. Additionally, the genes expression levels of carbohydrate-active enzymes (CAZymes) and cell wall degrading enzymes (CWDEs) were significantly increased at the late infection stage. Furthermore, we identified 807 potential secreted proteins and 78 small cysteine-rich proteins, which may function as fungal effectors and involved in the pathogenicity. These results provide valuable insights into critical and potential genes as well as the pathways involved in the pathogenesis of R. solani AG-3 TB.

Keywords: Rhizoctonia solani AG-3 TB; carbohydrate-active enzymes; cell wall degrading enzymes; effector; pathogenic molecular mechanism; secondary metabolites.