Elevated CO2 exacerbates effects of TiO2 nanoparticles on rice (Oryza sativa L.) leaf transcriptome and soil bacteria

Wenqian Ma; Wenchao Du; Kaihua Gu; Meiling Xu; Ying Yin; Yuanyuan Sun; Jichun Wu; Jianguo Zhu; Hongyan Guo

doi:10.1016/j.scitotenv.2022.159689

Elevated CO₂ exacerbates effects of TiO₂ nanoparticles on rice (Oryza sativa L.) leaf transcriptome and soil bacteria

Sci Total Environ. 2023 Jan 20;857(Pt 2):159689. doi: 10.1016/j.scitotenv.2022.159689. Epub 2022 Oct 24.

Authors

Wenqian Ma¹, Wenchao Du², Kaihua Gu¹, Meiling Xu³, Ying Yin¹, Yuanyuan Sun⁴, Jichun Wu⁴, Jianguo Zhu⁵, Hongyan Guo¹

Affiliations

¹ State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210023, China.
² School of Environment, Nanjing Normal University, Nanjing 210023, China. Electronic address: du@njnu.edu.cn.
³ College of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, China.
⁴ State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Surficial Geochemistry, School of Earth Sciences and Engineering, Nanjing University, Nanjing 210023, China.
⁵ State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science, Nanjing 210008, China.

PMID: 36302435
DOI: 10.1016/j.scitotenv.2022.159689

Abstract

Elevated CO₂ affects the plant rhizosphere and can therefore affect the fate and toxicity of soil contaminants. However, little is known about how the effects of nanoparticles on plants and soil bacteria will change under future CO₂ levels. A free-air CO₂ enrichment system with two CO₂ levels (ambient, 390 μmol mol^-1; elevated, 590 μmol mol^-1) was used to investigate the responses of rice (Oryza sativa L.) and soil bacteria to titanium dioxide nanoparticles (nano-TiO₂, 0 and 200 mg kg^-1). Results showed that nano-TiO₂ alone did not significantly affect rice growth but affected soil bacteria involved in the carbon and sulfur cycles. Elevated CO₂ alone increased rice plant biomass and up-regulated genes related to ribosomes, but its combination with nano-TiO₂ down-regulated genes related to photosynthesis and photosynthetic antennae. Elevated CO₂ also exacerbated the disturbance by nano-TiO₂ to soil bacteria involved in carbon and nitrogen cycles, and consequently inhibited the rice growth. These findings provide a reference for the comprehensive evaluation for the risk of soil pollution.

Keywords: Bacteria community; Elevated CO(2); Rice cultivar; TiO(2) nanoparticle; Transcriptome.

MeSH terms

Bacteria
Carbon
Carbon Dioxide / analysis
Nanoparticles* / toxicity
Oryza*
Plant Leaves / chemistry
Soil
Transcriptome

Substances

Soil
titanium dioxide
Carbon Dioxide
Carbon