The Function of BBX Gene Family under Multiple Stresses in Nicotiana tabacum

Kangkang Song; Bin Li; Hui Wu; Yuxue Sha; Liting Qin; Xingyun Chen; Ying Liu; Heng Tang; Long Yang

doi:10.3390/genes13101841

The Function of BBX Gene Family under Multiple Stresses in Nicotiana tabacum

Genes (Basel). 2022 Oct 12;13(10):1841. doi: 10.3390/genes13101841.

Authors

Kangkang Song¹, Bin Li¹, Hui Wu^{2

3

4}, Yuxue Sha¹, Liting Qin¹, Xingyun Chen¹, Ying Liu¹, Heng Tang¹, Long Yang¹

Affiliations

¹ College of Plant Protection and Agricultural Big-Data Research Center, Shandong Agricultural University, Tai'an 271018, China.
² Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
³ Key Laboratory of Dryland Agriculture, Ministry of Agriculture and Rural Affairs, Beijing 100081, China.
⁴ AgricultureIsLife, Gembloux Agro-Bio Tech, Liege University, Passage des Déportés 2, 5030 Gembloux, Belgium.

Abstract

B-box (BBX) is a zinc finger transcription factor, which is involved in regulating the growth and development of plants and resisting various stresses. In this study, 43 NtBBX genes were identified and divided into five subgroups in tobacco. The members in each subgroup had similar characteristics. The promoter region of NtBBX genes had cis-acting elements related to light response, hormone regulation and stress response. Transcriptome analysis showed that NtBBX30 was significantly up-regulated, and NtBBX12, NtBBX13, NtBBX16 and NtBBX17 were significantly down-regulated under abiotic stresses. The NtBBX genes also responded to the infection of Ralstonia solanacearum. NtBBX9, NtBBX1, NtBBX15 and NtBBX17 showed the greatest response under stresses. The NtBBX genes are expressed in various degrees under different tissues. This research will provide a solid foundation for further study of the biological function of NtBBX genes in tobacco.

Keywords: BBX gene family; Nicotiana tabacum; expression patterns; stress.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Regulation, Plant* / genetics
Hormones
Nicotiana* / genetics
Nicotiana* / metabolism
Plant Proteins / metabolism
Transcription Factors / metabolism

Substances

Plant Proteins
Transcription Factors
Hormones

Grants and funding

This work was supported by the Foundation of Shandong Province Modern Agricultural Technology System Innovation Team (SDAIT-25-01) and Taishan brand cigarette high-quality core raw material development and application in Shandong (202102004).