De Novo Assembly Transcriptome Analysis Reveals the Preliminary Molecular Mechanism of Primordium Formation in Pleurotus tuoliensis

Chunxia Wang; Jinkan Zhou; Zijian Cao; Bao Hu; Jing Wang; Jinying Guo; Suyue Zheng

doi:10.3390/genes13101747

De Novo Assembly Transcriptome Analysis Reveals the Preliminary Molecular Mechanism of Primordium Formation in Pleurotus tuoliensis

Genes (Basel). 2022 Sep 27;13(10):1747. doi: 10.3390/genes13101747.

Authors

Chunxia Wang¹, Jinkan Zhou¹, Zijian Cao¹, Bao Hu¹, Jing Wang¹, Jinying Guo¹, Suyue Zheng¹

Affiliation

¹ School of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056038, China.

Abstract

Primordium formation is extremely important for yield of Pleurotus tuoliensis. However, the molecular mechanism underlying primordium formation is largely unknown. This study investigated the transcriptional properties during primordium formation of P. tuoliensis by comparing transcriptome. Clean reads were assembled into 57,075 transcripts and 6874 unigenes. A total of 1397 differentially expressed genes were identified (26 DEGs altered in all stages). GO and KEGG enrichment analysis showed that these DEGs were involved in "oxidoreductase activity", "glycolysis/gluconeogenesis", "MAPK signaling pathways", and "ribosomes". Our results support further understanding of the transcriptional changes and molecular processes underlying primordium formation and differentiation of P. tuoliensis.

Keywords: Pleurotus tuoliensis; RNA-seq; molecular mechanism; primordium formation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Profiling
Oxidoreductases / genetics
Pleurotus* / genetics
Transcriptome / genetics

Substances

Oxidoreductases

Grants and funding

This work was supported by the Earmarked Fund for Hebei Edible Fungi Innovation Team of Modern Agro-industry Technology Research System (No. HBCT2018050202) and the Discipline Technology Research Program in Colleges and Universities of Hebei Province (No. QN2021210).