Expression Analysis of a Novel Oxidoreductase Glutaredoxin 2 in Black Tiger Shrimp, Penaeus monodon

Rui Fan; Yundong Li; Qibin Yang; Song Jiang; Jianhua Huang; Lishi Yang; Xu Chen; Falin Zhou; Shigui Jiang

doi:10.3390/antiox11101857

Expression Analysis of a Novel Oxidoreductase Glutaredoxin 2 in Black Tiger Shrimp, Penaeus monodon

Antioxidants (Basel). 2022 Sep 20;11(10):1857. doi: 10.3390/antiox11101857.

Authors

Rui Fan^{1

2}, Yundong Li¹, Qibin Yang^{1

3}, Song Jiang¹, Jianhua Huang¹, Lishi Yang¹, Xu Chen^{1

3}, Falin Zhou^{1

3}, Shigui Jiang¹

Affiliations

¹ Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
² College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
³ Tropical Fishery Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China.

Abstract

Glutaredoxin (Grx) is a glutathione-dependent oxidoreductase that is an important component of the redox system in organisms. However, there is a serious lack of sequence information and functional validation related to Grx in crustaceans. In this study, a novel Grx was identified in Penaeus monodon (PmGrx2). The full-length cDNA of PmGrx2 is 998 bp, with an open reading frame (ORF) of 441 bp, encoding 119 amino acids. Sequence alignment showed that PmGrx2 had the highest identity with Grx2 of Penaeus vannamei at 96.64% and clustered with Grx2 of other crustaceans. Quantitative real-time PCR (qRT-PCR) analysis showed that PmGrx2 was expressed in all examined tissues, with higher expression levels in the stomach and testis. PmGrx2 was continuously expressed during development and had the highest expression level in the zygote stage. Both ammonia-N stress and bacterial infection could differentially induce the expression of PmGrx2 in hepatopancreas and gills. When PmGrx2 was inhibited, the expression of antioxidant enzymes was suppressed, the degree of apoptosis increased, and the GSH content decreased with the prolongation of ammonia-N stress. Inhibition of PmGrx2 resulted in shrimp being exposed to a greater risk of oxidative damage. In addition, an SNP locus was screened on the exons of PmGrx2 that was significantly associated with an ammonia-N-stress-tolerance trait. This study suggests that PmGrx2 is involved in redox regulation and plays an important role in shrimps' resistance to marine environmental stresses.

Keywords: Penaeus monodon; SNPs; glutaredoxin; oxidative stress.

Grants and funding

This research was funded by Research and Development Projects in Key Areas of Guangdong Province, grant number 2021B0202003; China Agriculture Research System of MOF and MARA, grant number CARS-48; Central Public-interest Scientific Institution Basal Research Fund, CAFS, grant number No. 2020TD30; Financial Fund of Ministry of Agriculture and Rural affairs of Chin, grant number NHYYSWZZZYKZX2020; Central Public Interest Scientific Institution Basal Research Fund, South China Sea Fisheries Research Institute, CAFS, grant number NO.2021SD13; and Guangdong Basic and Applied Basic Research Foundation, grant number 2020A1515110200.