Fabrication, characterization of carboxymethyl konjac glucomannan/ovalbumin-naringin nanoparticles with improving in vitro bioaccessibility

Weimin Tang; Yanjun Wei; Wenjing Lu; Di Chen; Qin Ye; Cen Zhang; Yufeng Chen; Chaogeng Xiao

doi:10.1016/j.fochx.2022.100477

Fabrication, characterization of carboxymethyl konjac glucomannan/ovalbumin-naringin nanoparticles with improving in vitro bioaccessibility

Food Chem X. 2022 Oct 13:16:100477. doi: 10.1016/j.fochx.2022.100477. eCollection 2022 Dec 30.

Authors

Weimin Tang^{1

2}, Yanjun Wei², Wenjing Lu², Di Chen², Qin Ye², Cen Zhang², Yufeng Chen^{1

3}, Chaogeng Xiao²

Affiliations

¹ National Engineering Research Center for Optical Instruments, College of Optical Science and Engineering, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, Zhejiang 310052, China.
² State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Food Science Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China.
³ College of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.

Abstract

Naringin is potential functional and therapeutic ingredient, has low bioavailability because of poor aqueous solubility. In this study, an ovalbumin (OVA)-carboxymethyl konjac glucomannan (CKGM) nano-delivery system was developed to enhance the bioavailability of naringin. The effects of proportion (OVA: CKGM), pH and naringin concentration were studied on the formation, encapsulation efficiency (EE) and bioaccessibility of OVA/CKGM-Naringin nanoparticles (OVA/CKGM-Naringin NPs). Its morphology and size were viewed by Scanning Electron Microscope (SEM) and Transmission Electron Microscopy (TEM). The cross-linkage between OVA and CKGM was verified by Fourier Transform Infrared Spectroscopy (FTIR) and Fluorescence Intensity analysis. The size of OVA/CKGM-Naringin NPs were 463.83 ± 18.50 nm (Polydispersity Index-PDI, 0.42 ± 0.05). It indicated that 2:1 of OVA: CKGM, pH 3 and 7 mg/mL of naringin concentration were optimized processing parameters of OVA/CKGM-Naringin NPs with EE (97.90 ± 2.97 %) and remarkably improved bioaccessibility (85.01 ± 2.52 %). The OVA/CKGM-Naringin NPs was energy efficiently prepared and verified as an ideal carrier of naringin.

Keywords: Bioavailability Enhancement; Encapsulation efficiency (EE); Flavanone; Fluorescence Intensity; Nano-delivery system; Water Solubility.