Rapid and sensitive detection of tenuazonic acid in agricultural by-products based on gold nano-flower lateral flow

Peiyuan Cai; Rongzhi Wang; Sumei Ling; Shihua Wang

doi:10.3389/fbioe.2022.1021758

Rapid and sensitive detection of tenuazonic acid in agricultural by-products based on gold nano-flower lateral flow

Front Bioeng Biotechnol. 2022 Oct 7:10:1021758. doi: 10.3389/fbioe.2022.1021758. eCollection 2022.

Authors

Peiyuan Cai^{1

2}, Rongzhi Wang¹, Sumei Ling¹, Shihua Wang¹

Affiliations

¹ State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
² College of Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou, China.

Abstract

Tenuazonic acid (TA) is a highly toxic mycotoxin mainly generated by the fungi of Alternaria genus and widely contaminates agricultural by-products. Given the threat of TA to food-security, it is very important to develop rapid and sensitive detection methods for TA monitoring. In this study, gold nano-particles (AuNP) with average diameter near 17.25 nm were prepared, and the developed AuNP-based strip has an assay time of 15 min with visual limit of detection (LOD) of 12.5 ng/ml and threshold of 100 ng/ml. To further improve sensitivity, multi-branched gold nano-flowers (AuNF) with average diameter near 50 nm were prepared and characterized by UV-VIS and TEM, and the established AuNF-based strip has visual LOD of 0.78 ng/ml and threshold of 50 ng/ml within 15 min. Both assays were applied to determine TA in apple juice and tomato ketchup, and the results were consistent with that of UHPLC-MS/MS. Thus, these assays could be applied for rapid determination of trace TA in real samples.

Keywords: TA; colloidal gold; lateral flow immunochromatographic assay; multi-branched gold nano-flower particle; mycotoxin; test strip.