3-Deoxysappanchalcone isolated from Caesalpinia sinensis shows anticancer effects on HeLa and PC3 cell lines: invasion, migration, cell cycle arrest, and signaling pathway

Dian Lv; Qi Lai; Qi Zhang; Ji-Hong Wang; Yuan-Ce Li; Guang-Zhi Zeng; Jun-Lin Yin

doi:10.1016/j.heliyon.2022.e11013

3-Deoxysappanchalcone isolated from Caesalpinia sinensis shows anticancer effects on HeLa and PC3 cell lines: invasion, migration, cell cycle arrest, and signaling pathway

Heliyon. 2022 Oct 12;8(10):e11013. doi: 10.1016/j.heliyon.2022.e11013. eCollection 2022 Oct.

Authors

Dian Lv¹, Qi Lai¹, Qi Zhang¹, Ji-Hong Wang¹, Yuan-Ce Li¹, Guang-Zhi Zeng¹, Jun-Lin Yin¹

Affiliation

¹ Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education; School of Ethnic Medicine, Yunnan Minzu University, Kunming, China.

Abstract

To study the antitumor activity of compound 3-desoxysulforaphane (3-DSC) isolated from Caesalpinia sinensis, SRB assay, clone formation assay, flow cytometric cell cycle assay, scratch assay, transwell assay, and molecular docking were used to investigate the inhibitory effect of 3-DSC on HeLa and PC3 cells. The results showed that 3-DSC inhibited the cell migration and invasion by down-regulating expression of N-cadherin, Vimentin, MMP-2, and MMP-9 in HeLa and PC3 cells; It also inhibits cell proliferation by promoting the expression of CDK1 (cyclin-dependent kinases 1) and CDK2 (cyclin-dependent kinases 2), which arrests the tumor cell cycle at G2 phase. 3-DSC inhibits phosphorylation of AKT and ERK and upregulates the expression of the tumor suppressor gene p53. Molecular docking results confirmed that 3-DSC could bind firmly to AKT. In conclusion, 3-DSC inhibited the proliferation, migration and invasion of HeLa and PC3 cells.

Keywords: Caesalpinia sinensis; Cell cycle; Invasion; Migration; Proliferation.