LIMS2 is Downregulated in Osteosarcoma and Inhibits Cell Growth and Migration

Chenying Su; Xiaona Cai; Taotao Xu; Yungang Wu; Licong Wang; Pinjie Chen; Chenxian Su

doi:10.1155/2022/4811260

LIMS2 is Downregulated in Osteosarcoma and Inhibits Cell Growth and Migration

J Oncol. 2022 Oct 13:2022:4811260. doi: 10.1155/2022/4811260. eCollection 2022.

Authors

Chenying Su¹, Xiaona Cai², Taotao Xu³, Yungang Wu¹, Licong Wang¹, Pinjie Chen¹, Chenxian Su⁴

Affiliations

¹ Department of Traditional Chinese Medicine Orthopedics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China.
² Department of Ultrasonography, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China.
³ Department of Orthopedics, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, China.
⁴ Department of Orthopedics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China.

Abstract

Methods: GEO, GEPIA, and UALCAN databases were used to assess LIMS2 expression in OS. UALCAN and CCLE databases were applied to assess the methylation levels of LIMS2 in OS tissues and cells, which was verified in OS cells using the methylation specific PCR. The effects of LIMS2 on regulating OS cell growth, migration and invasion were determined by CCK-8, Edu staining, and transwell chambers, respectively. The role of LIMS2 in the activation of MAPK signaling was assessed using western blotting assay in OS cells.

Results: LIMS2 expression was declined in OS tissues and cells, while its methylation level was increased. The low expression of LIMS2 was associated with shorter overall survival and disease-free survival. Overexpression of LIMS2 inhibited cell growth, migration, and invasion and decreased the levels of p-ERK/ERK, p-P38/P38, and p-JNK/JNK.

Conclusion: LIMS2 expression was decreased in OS, which was associated with hypermethylation level and poor prognosis. LIMS2 overexpression inhibited OS cell growth and migration, which may be caused by the suppression of MAPK signaling.