Co-analysis of cucumber rhizosphere metabolites and microbial PLFAs under excessive fertilization in solar greenhouse

Shuang Wang; Ting Bian; Tong Wu; Yidi Zhang; Muhammad Awais; Hongdan Fu; Zhouping Sun

doi:10.3389/fmicb.2022.1004836

Co-analysis of cucumber rhizosphere metabolites and microbial PLFAs under excessive fertilization in solar greenhouse

Front Microbiol. 2022 Oct 6:13:1004836. doi: 10.3389/fmicb.2022.1004836. eCollection 2022.

Authors

Shuang Wang^{1

2

3

4}, Ting Bian^{1

2

3

4}, Tong Wu^{1

2

3

4}, Yidi Zhang^{1

2

3

4}, Muhammad Awais^{1

2

3

4}, Hongdan Fu^{1

2

3

4}, Zhouping Sun^{1

2

3

4}

Affiliations

¹ College of Horticulture, Shenyang Agricultural University, Shenyang, China.
² Key Laboratory of Protected Horticulture of the Education Ministry, Liaoning, China.
³ National and Local Joint Engineering Research Center of Northern Horticultural Facilities Design and Application Technology, Shenyang, China.
⁴ Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang, China.

Abstract

Fertilizer application is the most common measure in agricultural production, which can promote the productivity of crops such as cucumbers, but the problem of excessive fertilization occurs frequently in solar greenhouses. However, the effects of fertilization levels on cucumber rhizosphere soil microbes and metabolites and their relationships are still unclear. In order to determine how fertilization levels affect the rhizosphere microenvironment, we set up four treatments in the solar greenhouse: no-fertilization (N0P0K0), normal fertilization (N1P1K1), slight excessive fertilization (N2P2K2), and extreme excessive fertilization (N3P3K3). The results showed that fertilization treatments significantly increased cucumber yield compared to no-fertilization, but, the yield of N3P3K3 was significantly lower than that of N1P1K1 and N2P2K2. Fertilization levels had significant effects on rhizosphere microorganisms, and pH, NH₄ ⁺-N and AP were the main environmental factors that affected the changes in microbial communities. The total PLFAs, the percentages of fungi and arbuscular mycorrhizal fungi (AMF) were significantly reduced and bacteria percentage was significantly increased in N3P3K3 compared to other fertilization treatments. Differential metabolites under different fertilization levels were mainly organic acids, esters and sugars. Soil phenols with autotoxic effect under fertilization treatments were higher than that of N0P0K0. In addition, compared with soil organic acids and alkanes of N0P0K0, N2P2K2 was significantly increased, and N3P3K3 was not significantly different. This suggested that cucumber could maintain microbial communities by secreting beneficial metabolites under slight excessive fertilization (N2P2K2). But under extremely excessive fertilization (N3P3K3), the self-regulating ability of cucumber plants and rhizosphere soil was insufficient to cope with high salt stress. Furthermore, co-occurrence network showed that 16:1ω5c (AMF) was positively correlated with 2-palmitoylglycerol, hentriacontane, 11-octadecenoic acid, decane,4-methyl- and d-trehalose, and negatively correlated with 9-octadecenoic acid at different fertilization levels. This indicated that the beneficial microorganisms in the cucumber rhizosphere soil promoted with beneficial metabolites and antagonized with harmful metabolites. But with the deepening of overfertilization, the content of beneficial microorganisms and metabolites decreased. The study provided new insights into the interaction of plant rhizosphere soil metabolites and soil microbiomes under the different fertilization levels.

Keywords: cucumber; excessive chemical fertilizer; metabolites; microbials PLFAs; rhizosphere; solar greenhouse.