In vivo evidence of the prevents DSS-induced colitis of Lactiplantibacillus plantarum L15

Zengbo Wang; Liu Yang; Hongwei Tang; Kangyong Zhang; Qingxue Chen; Caihua Liu; Yanan Guo; Minghao Li; Zengwang Guo; Bailiang Li

doi:10.3389/fmicb.2022.1028919

In vivo evidence of the prevents DSS-induced colitis of Lactiplantibacillus plantarum L15

Front Microbiol. 2022 Oct 6:13:1028919. doi: 10.3389/fmicb.2022.1028919. eCollection 2022.

Authors

Zengbo Wang^{1

2}, Liu Yang^{1

2}, Hongwei Tang^{1

2}, Kangyong Zhang^{1

2}, Qingxue Chen^{1

2}, Caihua Liu¹, Yanan Guo¹, Minghao Li¹, Zengwang Guo¹, Bailiang Li^{1

2}

Affiliations

¹ Food College, Northeast Agricultural University, Harbin, China.
² Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin, China.

Abstract

Ulcerative colitis (UC) is challenging to treat and severely impacts patients and families. A previous study reported immunomodulatory and reduction of pro-inflammatory properties for the Lactiplantibacillus plantarum L15. This study aimed to analyze the preventive properties and mechanistic actions in an in vivo colitis model. The histopathological alteration, inflammation cytokines, and intestinal barrier function were analyzed. Subsequently, the cecal gut microbiota contents and products from different groups were detected. Finally, gene expressions related to the NF-κB signaling process were evaluated. L. plantarum L15 significantly decreased disease activity index (DAI), myeloperoxidase activity (MPO), pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) level, and increased weight change, colon length, and production of inflammation-suppressing cytokines. Furthermore, this strain supplementation substantially increased ZO-1, Occludin, and Claudin-1, and MUC2 mRNA expression levels with a corresponding decrease in serum lipopolysaccharide and D-lactic acid contents. In addition, L. plantarum L15 improved gut microbiota composition and increased short-chain fatty acid (SCFAs) in the colon content, which significantly reduced the transfer of NF-κB p65 to the nucleus. Our findings provide a theoretical basis for L. plantarum L15 as a preventive candidate for UC.

Keywords: Lactiplantibacillus plantarum; NF-κB signaling pathway; SCFAs; gut microbiota composition; inflammatory response; intestinal integrity.