Nanozymes show great potential as broad-spectrum antibacterial agents in the field of anti-infection. Their feasibility of application has received great hindrance due to low catalytic performance, insufficient reactive oxygen species (ROS), complex material design, and biosafety issues. Herein, we discovered an Fe3+-centered Melanoidin/Fe3+ nanozyme that exhibited superior reaction rate and catalytic activity to horseradish peroxidase (HRP) through the carboxyl, nitrogenous structure of Melanoidin. Melanoidin/Fe3+ catalyzed the formation of hydrogen peroxide (H2O2) into highly toxic superoxide anion (O2-•), which promoted the antibacterial ability of H2O2. The results showed that Melanoidin/Fe3+ had an excellent antibacterial effect against methicillin-resistant Staphylococcus aureus (MRSA) in low concentration H2O2 system. Both the infection wound model experiment and the biocompatibility experiment showed that the Melanoidin/Fe3+ promoted wound healing and had impressive biosafety. Thus, Melanoidin/Fe3+ shows ideal promise for the design of artificial enzymes with catalytic properties comparable to those of natural enzymes and clinical antibacterial therapy.
Keywords: Antibacterial therapy; MRSA; Melanoidin/Fe(3+); Peroxidase-like activity; Superoxide anion.
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