Present study is aimed to evaluate the influence of earthworm (Eisenia fetida), Cyanobacteria (Cylindrospermum stagnale), and rice husk biochar (BC) on cadmium (Cd) detoxification in artificially contaminated soil. The Cd content was kept at 10 mg/kg in factorial design I, coupled with 2% and 0% BC. E. fetida and C. stagnale un-inoculated and inoculated experiments were maintained respectively as negative and positive controls. In factorial design II, E. fetida and C. stagnale were inoculated, along with BC (0% and 2%, denoted as B), without BC (WB), along with four different Cd concentrations (Cd-0, Cd-5, Cd-10, and Cd-20 mg/kg). Results suggest a substantial amount of Cd removal in BC-assisted treatments when compared to negative control-1. Cd (mg/g) in E. fetida tissue ranged from 0.019 (WB2) to 0.0985 (B4). C. stagnale of WB4 (0.036) bioaccumulated the most Cd (mg/g), while B2 showed the least (0.018). The maximum quantity of metallothionein (5.34 μM/mg) was detected in E. fetida of B4 (factorial design - II) and the minimum was claimed in WB1 (0.48 μM/mg) at the end. Earthworm metallothionein protein is a key component in Cd removal from soil by playing an important role in detoxification process. Microbial communities and humic substances were observed in BC-assisted treatments, which aided in Cd-contaminated soil remediation. The present findings suggest that BC (2%) + earthworms + algae could be a suitable remediation strategy for Cd contaminated soil. BC + earthworm + algal-based investigation on heavy metal remediation will be a valuable platform for detoxifying harmful metals in soils.
Keywords: Biochar; Cadmium; Cylindrospermum stagnale; Earthworm; Metal binding protein.
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