Wound healing activity of aqueous dispersion of fullerene C60 produced by "green technology"

N N Shershakova; S M Andreev; A A Tomchuk; E A Makarova; A A Nikonova; E A Turetskiy; O A Petukhova; O Y Kamyshnikov; O I Ivankov; O A Kyzyma; O V Tomchuk; M V Avdeev; A S Dvornikov; D A Kudlay; M R Khaitov

doi:10.1016/j.nano.2022.102619

Wound healing activity of aqueous dispersion of fullerene C₆₀ produced by "green technology"

Nanomedicine. 2023 Jan:47:102619. doi: 10.1016/j.nano.2022.102619. Epub 2022 Oct 19.

Authors

N N Shershakova¹, S M Andreev², A A Tomchuk³, E A Makarova², A A Nikonova², E A Turetskiy², O A Petukhova², O Y Kamyshnikov², O I Ivankov⁴, O A Kyzyma⁵, O V Tomchuk⁶, M V Avdeev³, A S Dvornikov⁷, D A Kudlay⁸, M R Khaitov⁹

Affiliations

¹ NRC Institute of Immunology FMBA of Russia, Kashirskoe shosse, 24, Moscow 115522, Russian Federation. Electronic address: nn.shershakova@nrcii.ru.
² NRC Institute of Immunology FMBA of Russia, Kashirskoe shosse, 24, Moscow 115522, Russian Federation.
³ International intergovernmental organization Joint Institute for Nuclear Research, st. Joliot-Curie, 6, Dubna, Moscow Region 141980, Russian Federation.
⁴ International intergovernmental organization Joint Institute for Nuclear Research, st. Joliot-Curie, 6, Dubna, Moscow Region 141980, Russian Federation; Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region 141701, Russian Federation.
⁵ Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Street, Kyiv 0160, Ukraine.
⁶ International intergovernmental organization Joint Institute for Nuclear Research, st. Joliot-Curie, 6, Dubna, Moscow Region 141980, Russian Federation; Taras Shevchenko National University of Kyiv, 64/13, Volodymyrska Street, Kyiv 0160, Ukraine.
⁷ Pirogov Russian National Research Medical University, 1 Ostrovityanov St., Moscow 119997, Russian Federation.
⁸ NRC Institute of Immunology FMBA of Russia, Kashirskoe shosse, 24, Moscow 115522, Russian Federation; I.M. Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya str., Moscow 119991, Russian Federation.
⁹ NRC Institute of Immunology FMBA of Russia, Kashirskoe shosse, 24, Moscow 115522, Russian Federation; Pirogov Russian National Research Medical University, 1 Ostrovityanov St., Moscow 119997, Russian Federation.

PMID: 36272619
DOI: 10.1016/j.nano.2022.102619

Abstract

In addition to exhibited antioxidant and anti-inflammatory activity, fullerene C60 is a promising wound healing agent. An important stage in the production of fullerene-based ointments is the stability of the aqueous fullerene dispersion (AFD) with minimum size of colloidal fullerene aggregates and sufficiently high concentration. To achieve these parameters tangential flow filtration of fullerene C60 was used ("green technology"). As estimated by small-angle neutron scattering and dynamic light scattering purified AFDs with narrow-size distribution nanoclusters have a size of 6 nm and are assembled into agglomerates which reach a size of 150 nm. The ability of the AFD to exhibit regenerative activity was studied using the animal wound model. This study shows for the first time that the fullerene-based composition stimulates the healing of wounds of various origins. We assume that the mechanism of the AFD wound-healing activity is associated with the aryl hydrocarbon receptor and macrophages activity.

Keywords: AhR; Dynamic light scattering; Fullerene C(60); Green technology AFD; Regenerative activity; Small-angle scattering.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Technology*