Baicalin inhibits oxidative injures of mouse uterine tissue induced by acute heat stress through activating the Keap1/Nrf2 signaling pathway

Abstract

Heat stress effect the physiological functions of body, and reproductive system is one of the most sensitive. It's imperative to find out suitable measures to alleviate harmful effects of heat stress. Baicalin is well-known with its antioxidative property. To examine whether Baicalin could reduce oxidative injures of uterine tissue in heat-stressed mice. The mice were divided into four groups: control (Con), Baicalin (Bai), heat stress (H) and heat stress plus Baicalin (H + Bai). The oxidative damage of uterine tissue was detected by ELISA, H&E staining, tunnel assay and immunohistochemical staining. The protein/mRNA expressions of Keap1/Nrf2 related factors were detected by Western blot or QPCR. The results showed that mice heat-stressed at 41 °C for 2 h induced macroscopic changes, significantly increased MDA content and reduced activities of antioxidant enzymes including SOD, CAT and GSH-Px of the uterine tissue. Compared with Con group, heat stress up-regulated caspase-3 and caspase-9, enhanced the apoptosis of endometrial epithelial and glandular epithelial cells, improved the HO-1 mRNA/protein and NQO1 protein expressions, while down-regulated the mRNA/protein of Keap1. Compared with H group, antioxidant enzyme activities, Nrf2 protein and Nrf2, NQO1 and GCLC mRNA expressions were significantly increased in the H + Bai group. While the uterine epithelial cells apoptosis, MDA contents, caspase-3, caspase-9 and Keap1 protein and HO-1 mRNA expressions were decreased in the H + Bai group of mice compared with that in H group. Briefly, acute heat stress causes oxidative injures and apoptosis of mouse uterine tissue and Baicalin protects uterine tissue from the damages possibly through Keap1/Nrf2 signaling pathway.

Keywords: Baicalin; Heat stress; Keap1/Nrf2 signaling pathway; Mouse uterine; Oxidative damage.